Variability and Bias in Microbiome Metagenomic Sequencing: an Interlaboratory Study Comparing Experimental Protocols
This repository provides analysis code and results produced during evaluation of metagenomic sequencing (MGS) data collected through the Mosaic Standards Challenge. The Mosaic Standards Challenge asked participating laboratories analyze the same set of 7 samples using their own favored MGS laboratory methods. Each lab submitted their raw sequencing results and protocol information. The resulting MGS data was analyzed through a common bioinformatic pipeline and then evaluated to determine the effects of methodological choices.
Complete Metadata
| @type | dcat:Dataset |
|---|---|
| accessLevel | public |
| accrualPeriodicity | irregular |
| bureauCode |
[
"006:55"
]
|
| contactPoint |
{
"fn": "Samuel P. Forry",
"hasEmail": "mailto:samuel.forry@nist.gov"
}
|
| description | This repository provides analysis code and results produced during evaluation of metagenomic sequencing (MGS) data collected through the Mosaic Standards Challenge. The Mosaic Standards Challenge asked participating laboratories analyze the same set of 7 samples using their own favored MGS laboratory methods. Each lab submitted their raw sequencing results and protocol information. The resulting MGS data was analyzed through a common bioinformatic pipeline and then evaluated to determine the effects of methodological choices. |
| identifier | ark:/88434/mds2-2939 |
| issued | 2023-03-02 |
| keyword |
[
"microbiome; methodology; metagenomic sequencing"
]
|
| landingPage | https://data.nist.gov/od/id/mds2-2939 |
| language |
[
"en"
]
|
| license | https://www.nist.gov/open/license |
| modified | 2023-02-03 00:00:00 |
| programCode |
[
"006:045"
]
|
| publisher |
{
"name": "National Institute of Standards and Technology",
"@type": "org:Organization"
}
|
| theme |
[
"Bioscience:Microbial measurements"
]
|
| title | Variability and Bias in Microbiome Metagenomic Sequencing: an Interlaboratory Study Comparing Experimental Protocols |
