Found 75 datasets matching "cDNA".
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Background Gene expression profiling among different tissues is of paramount interest in various areas of biomedical research. We have developed a novel method (DADA, Digital Analysis of...
Search relevance: 137.65 | Views last month: 1 -
Background High density cDNA microarray technology provides a powerful tool to survey the activity of thousands of genes in normal and diseased cells, which helps us both to understand...
Search relevance: 123.27 | Views last month: 0 -
Background PCR amplification of target molecules involves sequence specific primers that flank the region to be amplified. While this technique is generally routine, its applicability...
Search relevance: 120.48 | Views last month: 0 -
Background The centromere is a specialized locus that mediates chromosome movement during mitosis and meiosis. This chromosomal domain comprises a uniquely packaged form of...
Search relevance: 119.82 | Views last month: 0 -
Background Members of the ribonuclease III superfamily of double-stranded(ds)-RNA-specific endoribonucleases participate in diverse RNA maturation and decay pathways in eukaryotic and...
Search relevance: 116.39 | Views last month: 0 -
Background A key assumption in the analysis of microarray data is that the quantified signal intensities are linearly related to the expression levels of the corresponding genes. To test...
Search relevance: 100.90 | Views last month: 0 -
Parasitic helminths of the genusSchistosomamate, achieve sexual maturity and produce eggs in the bloodstream of their definitive hosts, and the most important pathological consequences of the...
Search relevance: 95.01 | Views last month: 0 -
Background The p16INK4A gene product halts cell proliferation by preventing phosphorylation of the Rb protein. The p16INK4a gene is often deleted in human glioblastoma multiforme,...
Search relevance: 78.49 | Views last month: 1 -
Background Suppression Subtractive Hybridization PCR (SSH PCR) is a sophisticated cDNA subtraction method to enrich and isolate differentially expressed genes. Despite its popularity,...
Search relevance: 76.36 | Views last month: 0 -
Background cDNA-RDA is one of the subtractive cloning techniques used to isolate differentially expressed genes between two complex cDNA populations. In the present study we present a...
Search relevance: 71.69 | Views last month: 0 -
Within the fold: assessing differential expression measures and reproducibility in microarray assays
Fold-change' cutoffs have been widely used in microarray assays to identify genes that are differentially expressed. More accurate measures are required to identify high-confidence sets of genes...
Search relevance: 65.56 | Views last month: 0 -
Background The objective of this research was to investigate the reproducibility of cross-species microarray hybridisation. Comparisons between same- and cross-species hybridisations...
Search relevance: 61.09 | Views last month: 0 -
Background: Classic methods of identifying genes involved in neural function include the laborious process of behavioral screening of mutagenized flies and then rescreening candidate...
Search relevance: 57.36 | Views last month: 0 -
A cell-culture model was used to establish a profile of gene expression during the effector phase of ceramide-mediated cell death. Of the 239 genes that met the criteria for differential...
Search relevance: 56.93 | Views last month: 0 -
Background Somatic cell mutants can be informative in the analysis of a wide variety of cellular processes. The use of map-based positional cloning strategies in somatic cell hybrids to...
Search relevance: 48.80 | Views last month: 1 -
Researchers sequenced 10,368 expressed sequence tags (EST) clones using a normalized cDNA library made from pooled samples of the trophont, tomont, and theront life-cycle stages, and generated...
Search relevance: 43.75 | Views last month: 4 -
Background Familial atrial fibrillation, an autosomal dominant disease, was previously mapped to chromosome 10q22. One of the genes mapped to the 10q22 region is DLG5, a member of the...
Search relevance: 42.54 | Views last month: 0 -
Background Protein-protein interactions have decisive roles in almost all aspects of the structural and functional organization of cells. But in spite of the increasing amount of...
Search relevance: 42.54 | Views last month: 0 -
Background Drug resistance in breast cancer is a major obstacle to successful chemotherapy. In this study we used cDNA microarray technology to examine gene expression profiles obtained...
Search relevance: 42.15 | Views last month: 6 -
Background Gene disruption by targeted integration of transfected constructs becomes increasingly popular for studies of gene function. The chicken B cell line DT40 has been widely used...
Search relevance: 42.15 | Views last month: 0
