Found 31 datasets matching "gene-targeting".
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Background Gene targeting would offer a number of advantages over current transposon-based strategies for insect transformation. These include freedom from both position effects...
Search relevance: 242.11 | Views last month: 0 -
Background Recent progress in insect transgenesis has been dramatic but existing transposon-based approaches are constrained by position effects and potential instability. Gene targeting...
Search relevance: 204.99 | Views last month: 0 -
Data were collected to determine the abundance of nitrogen-fixing microorganisms in Cladophora algae growing on rocks, breakwall structures, or submerged dreissenid mussel beds around southern...
Search relevance: 120.50 | Views last month: 0 -
Background Expression of transgenes in mice requires transcriptional regulatory elements that direct expression in a chosen cell type. Unfortunately, the availability of...
Search relevance: 101.04 | Views last month: 0 -
Background A third DNA strand can bind into the major groove of a homopurine duplex DNA to form a DNA triple helix. Sequence specific triplex formation can be applied for gene targeting,...
Search relevance: 97.80 | Views last month: 0 -
Background The multisubunit (α1S,α2-δ, β1a and γ1) skeletal muscle dihydropyridine receptor (DHPR) transduces membrane depolarization into release of Ca2+ from the sarcoplasmic reticulum...
Search relevance: 90.25 | Views last month: 0 -
Background SFHR (small fragment homologous replacement)-mediated targeting is a process that has been used to correct specific mutations in mammalian cells. This process involves both...
Search relevance: 61.29 | Views last month: 0 -
An intense suppression of T cell proliferation to mitogens and to antigens is observed in a large number of parasitic infections. The impairment of T cell proliferation also occurred during...
Search relevance: 55.71 | Views last month: 0 -
The dataset contains sequencing read counts of fish prey eDNA metabarcoding (using primers targeting the 16S rRNA mitochondrial gene) that were extracted from fecal and cloacal swab samples...
Search relevance: 52.81 | Views last month: 0 -
Respirable antisense oligonucleotides (RASONs), which attenuate specific disease-associated mRNAs, represent a new class of respiratory therapeutics with considerable potential. RASONs overcome...
Search relevance: 49.74 | Views last month: 1 -
These data describe delivery of novel gene silencing agents (GSAs) to leaf tissue of the invasive common reed <i>Phragmites australis</i> ssp. <i>australis</i> using cell penetrating peptides...
Search relevance: 49.55 | Views last month: 0 -
Exposure to radiation provokes cellular responses controlled in part by gene expression networks. MicroRNAs (miRNAs) are small non-coding RNAs which mostly regulate gene expression by degrading...
Search relevance: 45.21 | Views last month: 0 -
Background Mucolipidosis type IV (MLIV) is an autosomal recessive lysosomal storage disorder characterized by severe neurologic and ophthalmologic abnormalities. Recently the MLIV gene,...
Search relevance: 44.55 | Views last month: 1 -
Background Transgenic mice have been used extensively to analyze gene function. Unfortunately, traditional transgenic procedures have only limited use in analyzing alleles that cause...
Search relevance: 44.42 | Views last month: 0 -
Exposure to radiation provokes cellular responses controlled in part by gene expression networks. MicroRNAs (miRNAs) are small non-coding RNAs which mostly regulate gene expression by degrading...
Search relevance: 43.85 | Views last month: 0 -
We introduce a new high-throughput transcriptomics (HTTr) platform comprised of a collagen sandwich primary rat hepatocyte culture and the TempO-Seq assay for screening and prioritizing potential...
Search relevance: 43.79 | Views last month: 5 -
Background Several Cre reporter strains of mice have been described, in which a lacZ gene is turned on in cells expressing Cre recombinase, as well as their daughter cells, following...
Search relevance: 43.38 | Views last month: 0 -
This dataset accompanies the manuscript Detection and differentiation of low virulence and virulent Orthoavulavirus javaense using a molecular beacon with RT-LAMP | Scientific Reports (nature.com)...
Search relevance: 39.38 | Views last month: 1 -
Background In mammals, L-threonine is an indispensable amino acid. The conversion of L-threonine to glycine occurs through a two-step biochemical pathway involving the enzymes...
Search relevance: 39.28 | Views last month: 0 -
Background Interest in methanogens from ruminants has resulted from the role of methane in global warming and from the fact that cattle typically lose 6 % of ingested energy as methane....
Search relevance: 38.41 | Views last month: 0
