Found 354 datasets matching "pcr".
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Background Chlamydia pneumoniae infection has been detected by serological methods, but PCR is gaining more interest. A number of different PCR assays have been developed and some are...
Search relevance: 145.26 | Views last month: 0 -
Background In a variant of the standard PCR reaction termed bridging, or jumping, PCR the primer-bound sequences are originally on separate template molecules. Bridging can occur if, and...
Search relevance: 127.76 | Views last month: 0 -
Background Leptospirosis is an emerging infectious disease. The differential diagnosis of leptospirosis is difficult due to the varied and often "flu like" symptoms which may result in a...
Search relevance: 122.52 | Views last month: 3 -
Dataset includes repeated measures of the National Institute of Standards and Technology Standard Reference Material 2917 (SRM 2917) using an Enterococcus digital PCR (dPCR) assay. “This...
Search relevance: 118.99 | Views last month: 2 -
Background Suppression Subtractive Hybridization PCR (SSH PCR) is a sophisticated cDNA subtraction method to enrich and isolate differentially expressed genes. Despite its popularity,...
Search relevance: 118.64 | Views last month: 0 -
Trypanosoma (Herpetosoma) lewisi is a trypanosome of the sub-genus Herpetosoma (Stercoraria section), parasite of rats (Rattus rattus and Rattus norvegicus) transmitted by fleas. T. lewisi has a...
Search relevance: 117.95 | Views last month: 0 -
Bacillus species and B. anthracis presence/absence data were determined in 4,770 soil samples collected across the contiguous United States, in cooperation with the U.S. Environmental Protection...
Search relevance: 111.66 | Views last month: 0 -
Background Prompt laboratory diagnosis of Herpes simplex virus (HSV) infection facilitates patient management and possible initiation of antiviral therapy. In our laboratory, which...
Search relevance: 109.59 | Views last month: 0 -
Background Norwalk-like viruses are the most common cause of gastroenteritis outbreaks and sporadic cases of vomiting and diarrhoea. In healthy individuals infection is often mild and...
Search relevance: 106.86 | Views last month: 0 -
Background Rapid diagnosis and correct treatment of cases are the main objectives of control programs in malaria-endemic areas. Methods and results ...
Search relevance: 104.55 | Views last month: 2 -
This dataset includes the standard curves for ICCRTqPCR to convert the assay quantities to the concentrations of infectious viruses and all the calculations on inactivation rate constants. Also,...
Search relevance: 103.19 | Views last month: 0 -
We developed and validated conventional and quantitative real-time PCR assays for the detection of DNA from the myxozoan parasite Tetracapsuloides bryosalmonae, the causative agent of...
Search relevance: 101.28 | Views last month: 1 -
Using real-time reverse transcription PCR ten housekeeping genes from different abundance and functional classes in various human tissues were evaluated. The conventional use of a single gene for...
Search relevance: 99.44 | Views last month: 5 -
Background We describe an alternative method to determine mRNA half-life (t1/2) based on the Real-Time RT-PCR procedure. This approach was evaluated by using the β-actin gene as a...
Search relevance: 93.26 | Views last month: 0 -
The entire process of reverse transcription-polymerase chain reaction (RT-PCR) is simplified by using oligonucleotide-immobilized microplates made of, e.g., polypropylene, to which...
Search relevance: 87.99 | Views last month: 0 -
The emergence of ophidiomycosis (or snake fungal disease) in snakes has prompted increased awareness of the potential impacts of fungal infections on wild reptile populations. Yet, aside from...
Search relevance: 87.55 | Views last month: 0 -
The dataset provides the raw data in support of Tables 1-4 of the paper and include: colony forming unit (CFU) data for each of three replicates of sand, loam, and clay soils; results for the...
Search relevance: 83.93 | Views last month: 0 -
Provides droplet digital polymerase chain reaction (ddPCR) and quantitative PCR (qPCR) measurements of adenovirus and norovirus in three decentralized wastewater and graywater collection systems....
Search relevance: 83.25 | Views last month: 0 -
A GeXP multiplex, RT-PCR assay was developed and optimized that simultaneously measures expression of a suite of immune-relevant genes in rainbow trout (Oncorhynchus mykiss), concentrating on...
Search relevance: 82.48 | Views last month: 0 -
Droplet digital PCR (ddPCR) is being advocated as a reference method to measure rare genomic targets. It has consistently been proven to be more sensitive and direct at discerning copy numbers of...
Search relevance: 81.39 | Views last month: 0
