Found 62 datasets matching "rt-pcr".
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Background Norwalk-like viruses are the most common cause of gastroenteritis outbreaks and sporadic cases of vomiting and diarrhoea. In healthy individuals infection is often mild and...
Search relevance: 212.74 | Views last month: 0 -
Background We describe an alternative method to determine mRNA half-life (t1/2) based on the Real-Time RT-PCR procedure. This approach was evaluated by using the β-actin gene as a...
Search relevance: 197.95 | Views last month: 0 -
The entire process of reverse transcription-polymerase chain reaction (RT-PCR) is simplified by using oligonucleotide-immobilized microplates made of, e.g., polypropylene, to which...
Search relevance: 179.62 | Views last month: 0 -
A GeXP multiplex, RT-PCR assay was developed and optimized that simultaneously measures expression of a suite of immune-relevant genes in rainbow trout (Oncorhynchus mykiss), concentrating on...
Search relevance: 169.84 | Views last month: 0 -
There is a need for development of an analytical method for rapid detection of SARS-CoV-2 virus which is causing the COVID-19 pandemic. Currently available traditional tissue/cell culture-based...
Search relevance: 161.62 | Views last month: 0 -
Background Suboptimal specimen processing and storage conditions of samples which contain hepatitis C virus (HCV) RNA may result in a decline of HCV RNA concentration or false-negative...
Search relevance: 143.53 | Views last month: 1 -
Using real-time reverse transcription PCR ten housekeeping genes from different abundance and functional classes in various human tissues were evaluated. The conventional use of a single gene for...
Search relevance: 137.71 | Views last month: 5 -
The dataset shows the results of the Running Fisher test between the TGx-DDI biomarker and each of the qPCR-generated gene lists from chemical treatments in TK6 cells. The results are divided into...
Search relevance: 132.98 | Views last month: 0 -
Background Abnormal collagen metabolism is thought to play an important role in the development of primary inguinal hernia. This is underlined by detection of altered collagen metabolism...
Search relevance: 116.16 | Views last month: 0 -
This dataset includes the standard curves for ICCRTqPCR to convert the assay quantities to the concentrations of infectious viruses and all the calculations on inactivation rate constants. Also,...
Search relevance: 99.97 | Views last month: 0 -
Background Mutation analysis of the neurofibromatosis type 1 (NF1) gene has shown that about 30% of NF1 patients carry a splice mutation resulting in the production of one or several...
Search relevance: 99.38 | Views last month: 0 -
From 2010-2018 we investigated the occurrence of avian influenza virus in wild birds in Iceland. A total of 6635 swabs samples were collected from wild birds or fecal material directly associated...
Search relevance: 81.27 | Views last month: 3 -
Background This study describes the functional interaction between the putative Ca2+ channel TRP4 and the cystic fibrosis transmembrane conductance regulator, CFTR, in mouse aorta...
Search relevance: 69.82 | Views last month: 0 -
In summer 2020, SARS-CoV-2 was detected on mink farms in Utah. An interagency One Health response was initiated to assess the extent of the outbreak and included sampling animals from or near...
Search relevance: 67.64 | Views last month: 1 -
RT-qPCR is used world-wide to test and trace the spread of SARS-CoV-2. “Extraction-less” or “direct” RT-PCR is an open-access qualitative method for SARS-CoV-2 detection from nasopharyngeal (NP)...
Search relevance: 64.26 | Views last month: 1 -
This data set provides biological information from of a captive colony of common vampire bats (wild-caught and born in captivity) that were part of an experimental study on vaccination. It...
Search relevance: 64.09 | Views last month: 0 -
Background Biochemical and histochemical studies have both previously indicated plasma membrane-associated carbonic anhydrase (CA) activity in hepatocytes which has been assumed to be CA...
Search relevance: 63.30 | Views last month: 0 -
EPA Method 1615 measures enteroviruses and noroviruses present in environmental and drinking waters. The viral ribonucleic acid (RNA) from water sample concentrates is extracted and tested for...
Search relevance: 60.83 | Views last month: 0 -
Background The development and activation of CD4+ helper T cell (Th) subsets with distinct patterns of unbalanced production of cytokines play an important part in infectious, allergic...
Search relevance: 60.81 | Views last month: 1 -
Results of sampling to determine the SARS-CoV-2 N gene levels in NYC DEP Wastewater Resource Recovery Facility (WRRF) influent, disaggregated by the WRRF where the sample was collected, date...
Search relevance: 60.53 | Views last month: 1
