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2018 Embarrass River Microsporidia
During an epidemiologic survey following a mortality event of freshwater mussels in 2018 in the Embarrass River, Wisconsin, USA, we identified a novel microsporidian parasite in the ovary of mucket (Actinonaias ligamentina), plain pocketbook (Lampsilis cardium), and fatmucket (Lampsilis siliquoidea) (Unioinidae). Histopathology showed round-to-oval microsporidial spores in the cytoplasm of oocytes in 60% (3/5) of mucket, 100% (4/4) of plain pocketbook and 50% (1/2) of fatmucket. On transmission electron microscopy, mature spores were round to oval, measured 4.13 +/- 0.64 µm (3.14–5.31) long by 2.88 +/-0.37 µm (2.36–3.68) wide. Spores had a thin electron dense exospore with a spiky coat, a thick electron lucent endospore, diplokaryotic nuclei, a polar vacuole, and 27–28 polar filaments arranged in 1 to 3 rows. Sequencing of the small subunit rRNA produced a 1356 bp sequence most similar to Pseudonosema cristatellae, and phylogenetic analysis grouped it with freshwater Neopereziida. The morphologic and ultrastructural characteristics did not match those of Pseudonosema sp. and a new genus and species, Hirsutonosema embarrassi n. gen., n. sp., were designated. Additional studies could evaluate host susceptibility, distribution, seasonality, transmission, and lethal or sub-lethal effects of this parasite to freshwater mussels.
Complete Metadata
| accessLevel | public |
|---|---|
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[
"010:12"
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| contactPoint |
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"fn": "Susan Knowles",
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"hasEmail": "mailto:sknowles@usgs.gov"
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|
| description | During an epidemiologic survey following a mortality event of freshwater mussels in 2018 in the Embarrass River, Wisconsin, USA, we identified a novel microsporidian parasite in the ovary of mucket (Actinonaias ligamentina), plain pocketbook (Lampsilis cardium), and fatmucket (Lampsilis siliquoidea) (Unioinidae). Histopathology showed round-to-oval microsporidial spores in the cytoplasm of oocytes in 60% (3/5) of mucket, 100% (4/4) of plain pocketbook and 50% (1/2) of fatmucket. On transmission electron microscopy, mature spores were round to oval, measured 4.13 +/- 0.64 µm (3.14–5.31) long by 2.88 +/-0.37 µm (2.36–3.68) wide. Spores had a thin electron dense exospore with a spiky coat, a thick electron lucent endospore, diplokaryotic nuclei, a polar vacuole, and 27–28 polar filaments arranged in 1 to 3 rows. Sequencing of the small subunit rRNA produced a 1356 bp sequence most similar to Pseudonosema cristatellae, and phylogenetic analysis grouped it with freshwater Neopereziida. The morphologic and ultrastructural characteristics did not match those of Pseudonosema sp. and a new genus and species, Hirsutonosema embarrassi n. gen., n. sp., were designated. Additional studies could evaluate host susceptibility, distribution, seasonality, transmission, and lethal or sub-lethal effects of this parasite to freshwater mussels. |
| distribution |
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| identifier | http://datainventory.doi.gov/id/dataset/USGS_660b1df3d34e4df16bd58c39 |
| keyword |
[
"Hirsutonosema embarrassi n. sp.",
"Lampsilus cardium",
"Neopereziida",
"USGS:660b1df3d34e4df16bd58c39",
"biota",
"histopathology",
"microsporidia",
"transmission electron microscopy",
"unionid",
"wildlife disease"
]
|
| modified | 2024-06-05T00:00:00Z |
| publisher |
{
"name": "U.S. Geological Survey",
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|
| spatial | -88.7985782, 44.7397921, -88.7984012, 44.7398997 |
| theme |
[
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|
| title | 2018 Embarrass River Microsporidia |
