Analytical Performance of Direct-to-Consumer Gut Microbiome Testing Services
This repository contains raw and intermediate data files as well as analysis code from the manuscript "Evaluating the Analytical Performance of Direct-to-Consumer Gut Microbiome Testing Services". In this analysis a pilot version of the homogenized whole human stool reference material was submitted as a sample to commercial direct to consumer microbiome testing companies. In addition, NIST conducted in house characterization by 16S amplicon sequencing and whole metagenomic sequencing (WMS) on the same sample. Raw sequencing data from the sequencing providers was not obtained for this study; however sequencing files (fastq) both 16S amplicon sequencing analysis and whole metagenomic sequencing (WMS) conducted at NIST are included. In addition, the fastq files from other donors that contributed to the pilot material and included in this analysis to represent biological diversity are also included in this record.
Complete Metadata
| @type | dcat:Dataset |
|---|---|
| accessLevel | public |
| accrualPeriodicity | irregular |
| bureauCode |
[
"006:55"
]
|
| contactPoint |
{
"fn": "Stephanie Servetas",
"hasEmail": "mailto:stephanie.servetas@nist.gov"
}
|
| description | This repository contains raw and intermediate data files as well as analysis code from the manuscript "Evaluating the Analytical Performance of Direct-to-Consumer Gut Microbiome Testing Services". In this analysis a pilot version of the homogenized whole human stool reference material was submitted as a sample to commercial direct to consumer microbiome testing companies. In addition, NIST conducted in house characterization by 16S amplicon sequencing and whole metagenomic sequencing (WMS) on the same sample. Raw sequencing data from the sequencing providers was not obtained for this study; however sequencing files (fastq) both 16S amplicon sequencing analysis and whole metagenomic sequencing (WMS) conducted at NIST are included. In addition, the fastq files from other donors that contributed to the pilot material and included in this analysis to represent biological diversity are also included in this record. |
| distribution |
[
{
"title": "WMS raw reads",
"format": "zip file contains fastq files within two folders depending on the donor",
"mediaType": "application/zip",
"description": "The folder contains fastq files for data generated by NIST. a full description can be found in the README document",
"downloadURL": "https://data.nist.gov/od/ds/mds2-3797/WMS_raw_reads.zip"
},
{
"title": "Statistical Models",
"format": "zip folder includes: r markdowns, rdata, csv, png",
"mediaType": "application/zip",
"description": "This folder contains all files needed for the statistical models generated during data analysis",
"downloadURL": "https://data.nist.gov/od/ds/mds2-3797/StatisticalModels.zip"
},
{
"title": "publication set",
"format": "zip folder includes: fastq files (for 16S), r markdowns, rdata, csv, xlsx",
"mediaType": "application/zip",
"description": "This folder contains code for two sets of data analyses (1) for NIST 16S amplicon sequencing and (2) for taxonomic profile comparisons in support of the evaluation of the analytical performance of DTC gut microbiome testing services conducted in R as well as input/output data files. a complete description can be found in the README file",
"downloadURL": "https://data.nist.gov/od/ds/mds2-3797/publication_set.zip"
},
{
"title": "README",
"mediaType": "text/plain",
"downloadURL": "https://data.nist.gov/od/ds/mds2-3797/READMEv2.txt"
}
]
|
| identifier | ark:/88434/mds2-3348 |
| issued | 2024-06-20 |
| keyword |
[
"16S amplicon sequencing",
"Metagenomics",
"Microbiome",
"NGS",
"WMS",
"gut microbiome",
"whole metagenomic sequencing"
]
|
| landingPage | https://data.nist.gov/od/id/mds2-3348 |
| language |
[
"en"
]
|
| license | https://www.nist.gov/open/license |
| modified | 2024-06-05 00:00:00 |
| programCode |
[
"006:045"
]
|
| publisher |
{
"name": "National Institute of Standards and Technology",
"@type": "org:Organization"
}
|
| replaces | ark:/88434/mds2-3348 |
| theme |
[
"Bioscience:Microbial measurements",
"Health:Precision medicine"
]
|
| title | Analytical Performance of Direct-to-Consumer Gut Microbiome Testing Services |
