Assessing the Impact of Wastewater Treatment Plant Effluent on Downstream Drinking Water-Source Quality Using a Zebrafish (Danio Rerio) Liver Cell-Based Metabolomics Approach
The datafile presents the data used to create the figures in the manuscript. These include: a) Two-component score plots of hydrophilic and lipophilic ZFL cell extracts analyzed with NMR and GC-MS, respectively; b) The t-test filtered difference spectra (exposed minus control) generated from binned 1H NMR spectra (0.5–5.0 ppm in chemical shift) of hydrophilic extracts of ZFL cells; c) Bar chart showing the fold change of 21 annotated metabolites from the lipophilic extracts that exhibited different normalized abundance at one or more of the six sites relative to the control as measured by GC-MS; d) The summed intensity values for significantly changed metabolites from the t-test filtered difference spectra for both hydrophilic and lipophilic extracts across six sampling sites; and e) Relative normalized abundances for metabolites identified in the cholesterol and fatty acid synthesis pathways from lipophilic ZFL extracts.
This dataset is associated with the following publication:
Zhen, H., D. Ekman, T. Collette, S. Glassmeyer, M. Mills, E. Furlong, D. Kolpin, and Q. Teng. Assessing the impact of wastewater treatment plant effluent on downstream drinking water-source quality using a zebrafish (Danio Rerio) liver cell-based metabolomics approach. WATER RESEARCH. Elsevier Science Ltd, New York, NY, USA, 145: 198-209, (2018).
Complete Metadata
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| contactPoint |
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"fn": "Susan Glassmeyer",
"hasEmail": "mailto:glassmeyer.susan@epa.gov"
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| describedBy | https://pasteur.epa.gov/uploads/10.23719/1502544/documents/Data%20Dictionary.docx |
| describedByType | application/vnd.openxmlformats-officedocument.wordprocessingml.document |
| description | The datafile presents the data used to create the figures in the manuscript. These include: a) Two-component score plots of hydrophilic and lipophilic ZFL cell extracts analyzed with NMR and GC-MS, respectively; b) The t-test filtered difference spectra (exposed minus control) generated from binned 1H NMR spectra (0.5–5.0 ppm in chemical shift) of hydrophilic extracts of ZFL cells; c) Bar chart showing the fold change of 21 annotated metabolites from the lipophilic extracts that exhibited different normalized abundance at one or more of the six sites relative to the control as measured by GC-MS; d) The summed intensity values for significantly changed metabolites from the t-test filtered difference spectra for both hydrophilic and lipophilic extracts across six sampling sites; and e) Relative normalized abundances for metabolites identified in the cholesterol and fatty acid synthesis pathways from lipophilic ZFL extracts. This dataset is associated with the following publication: Zhen, H., D. Ekman, T. Collette, S. Glassmeyer, M. Mills, E. Furlong, D. Kolpin, and Q. Teng. Assessing the impact of wastewater treatment plant effluent on downstream drinking water-source quality using a zebrafish (Danio Rerio) liver cell-based metabolomics approach. WATER RESEARCH. Elsevier Science Ltd, New York, NY, USA, 145: 198-209, (2018). |
| distribution |
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"title": "Datasets for WW2DW manuscript-Metabolomics-Athens-081418.xlsx",
"mediaType": "application/vnd.openxmlformats-officedocument.spreadsheetml.sheet",
"downloadURL": "https://pasteur.epa.gov/uploads/10.23719/1502544/Datasets%20for%20WW2DW%20manuscript-Metabolomics-Athens-081418.xlsx"
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| identifier | https://doi.org/10.23719/1502544 |
| keyword |
[
"cell culture",
"de facto reuse",
"drinking water",
"metabolomics",
"potable reuse",
"source water",
"wastewater",
"wastewater treatment plant effluent",
"water quality"
]
|
| license | https://pasteur.epa.gov/license/sciencehub-license.html |
| modified | 2018-09-13 |
| programCode |
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"020:096"
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| publisher |
{
"name": "U.S. EPA Office of Research and Development (ORD)",
"subOrganizationOf": {
"name": "U.S. Environmental Protection Agency",
"subOrganizationOf": {
"name": "U.S. Government"
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}
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|
| references |
[
"https://doi.org/10.1016/j.watres.2018.08.028"
]
|
| rights |
null
|
| title | Assessing the Impact of Wastewater Treatment Plant Effluent on Downstream Drinking Water-Source Quality Using a Zebrafish (Danio Rerio) Liver Cell-Based Metabolomics Approach |
