Ceratocystis DNA Detection in Frass During Caged Sampler Comparison
We designed two new samplers for monitoring airborne particulates, including fungal and fern spores and plant pollen, that rely on natural wind currents (Passive Environmental Sampler) or a battery operated fan (Active Environmental Sampler). Both samplers are modeled after commercial devices such as the Rotorod® and the Burkard® samplers, but are more economical and require less maintenance than commercial devices. We compared our two new samplers to Rotorod® samplers using Xyleborus spp. boring dust known to contain ROD causing pathogens. The comparison was done in a large outdoor field cage to determine relative effectiveness of the three samplers for capturing windblown boring dust. The dataset contains results of quantitative PCR (qPCR) tests to determine presence of Ceratocystis lukuohia, Ceratocystis huliohia, and Metrosideros polymorpha DNA in the samplers after release of boring dust over the course of twelve trials where position of samplers was rotated.
Complete Metadata
| accessLevel | public |
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[
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{
"fn": "Kylle Roy",
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|
| description | We designed two new samplers for monitoring airborne particulates, including fungal and fern spores and plant pollen, that rely on natural wind currents (Passive Environmental Sampler) or a battery operated fan (Active Environmental Sampler). Both samplers are modeled after commercial devices such as the Rotorod® and the Burkard® samplers, but are more economical and require less maintenance than commercial devices. We compared our two new samplers to Rotorod® samplers using Xyleborus spp. boring dust known to contain ROD causing pathogens. The comparison was done in a large outdoor field cage to determine relative effectiveness of the three samplers for capturing windblown boring dust. The dataset contains results of quantitative PCR (qPCR) tests to determine presence of Ceratocystis lukuohia, Ceratocystis huliohia, and Metrosideros polymorpha DNA in the samplers after release of boring dust over the course of twelve trials where position of samplers was rotated. |
| distribution |
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|
| identifier | http://datainventory.doi.gov/id/dataset/USGS_5c1c1c85e4b0708288c7ac14 |
| keyword |
[
"Ceratocystis",
"Environmental DNA",
"Environmental Sampler",
"Metrosideros polymorpha",
"Ohia lehua",
"ROD",
"Rapid Ohia Death",
"Spore Traps",
"USGS:5c1c1c85e4b0708288c7ac14",
"environment"
]
|
| modified | 2020-08-27T00:00:00Z |
| publisher |
{
"name": "U.S. Geological Survey",
"@type": "org:Organization"
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|
| spatial | -155.096827, 19.695607, -155.091505, 19.700617 |
| theme |
[
"Geospatial"
]
|
| title | Ceratocystis DNA Detection in Frass During Caged Sampler Comparison |
