Cyanobacteria, Cyanotoxin, Cyanotoxin Synthetase Gene, and other Water-Quality Data Collected from Five River Basins in the North Atlantic Appalachian Region, August through September, 2020
This data release contains cyanotoxin, chlorophyll-a, and pheophytin-a concentration, cyanobacterial genetics, phytoplankton community composition, and multiparameter sonde data collected from 20 sites in five northeastern United States river basins (Penobscot (ME), Santuit (MA), York (VA-WV), Salem (NJ), and Peconic (NY)). Solid Phase Adsorption Toxin Tracking (SPATT) passive samplers were deployed at all sites between August 31 and September 2, 2020, and retrieved after 7 days. Discrete water samples were collected when SPATTs were deployed, and at 2 sites (USGS station IDs 01670257, 0167014792), samples were also collected when the SPATTs were recovered. Sonde data were collected when deploying and retrieving the SPATTs. The SPATT samples were extracted and analyzed for cyanotoxins (anatoxin-a, cylindrospermopsin, microcystin, saxitoxin) by enzyme-linked immunosorbent assay (ELISA) and liquid chromatography tandem mass spectrometry (LC/MS/MS). Sonde data included measurements of water temperature, pH, specific conductance, dissolved oxygen, turbidity, chlorophyll, phycocyanin, and phycoerythrin fluorescence (in relative fluorescence units and micrograms per liter), and nitrate, however some sensors were not available at all locations. Discrete samples were analyzed for total cyanotoxin concentration (anatoxin-a, cylindrospermopsin, microcystin, saxitoxin) by ELISA and LC/MS/MS, chlorophyll-a, pheophytin-a, cyanotoxin synthetase genes, and phytoplankton community composition. Saxitoxin could not being analyzed by LC/MS/MS so an additional ELISA analysis was performed instead.
Complete Metadata
| accessLevel | public |
|---|---|
| bureauCode |
[
"010:12"
]
|
| contactPoint |
{
"fn": "J. M. Trevino",
"@type": "vcard:Contact",
"hasEmail": "mailto:jtrevino@usgs.gov"
}
|
| description | This data release contains cyanotoxin, chlorophyll-a, and pheophytin-a concentration, cyanobacterial genetics, phytoplankton community composition, and multiparameter sonde data collected from 20 sites in five northeastern United States river basins (Penobscot (ME), Santuit (MA), York (VA-WV), Salem (NJ), and Peconic (NY)). Solid Phase Adsorption Toxin Tracking (SPATT) passive samplers were deployed at all sites between August 31 and September 2, 2020, and retrieved after 7 days. Discrete water samples were collected when SPATTs were deployed, and at 2 sites (USGS station IDs 01670257, 0167014792), samples were also collected when the SPATTs were recovered. Sonde data were collected when deploying and retrieving the SPATTs. The SPATT samples were extracted and analyzed for cyanotoxins (anatoxin-a, cylindrospermopsin, microcystin, saxitoxin) by enzyme-linked immunosorbent assay (ELISA) and liquid chromatography tandem mass spectrometry (LC/MS/MS). Sonde data included measurements of water temperature, pH, specific conductance, dissolved oxygen, turbidity, chlorophyll, phycocyanin, and phycoerythrin fluorescence (in relative fluorescence units and micrograms per liter), and nitrate, however some sensors were not available at all locations. Discrete samples were analyzed for total cyanotoxin concentration (anatoxin-a, cylindrospermopsin, microcystin, saxitoxin) by ELISA and LC/MS/MS, chlorophyll-a, pheophytin-a, cyanotoxin synthetase genes, and phytoplankton community composition. Saxitoxin could not being analyzed by LC/MS/MS so an additional ELISA analysis was performed instead. |
| distribution |
[
{
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"title": "Digital Data",
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"accessURL": "https://doi.org/10.5066/P94LAHHM",
"mediaType": "application/http",
"description": "Landing page for access to the data"
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"title": "Original Metadata",
"format": "XML",
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"description": "The metadata original format",
"downloadURL": "https://data.usgs.gov/datacatalog/metadata/USGS.6464e4d3d34ec179a83da4aa.xml"
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|
| identifier | http://datainventory.doi.gov/id/dataset/USGS_6464e4d3d34ec179a83da4aa |
| keyword |
[
"Anatoxin-a",
"Chlorophyll-a",
"Cyanobacteria",
"Cylindrospermopsin",
"Dolby Pond",
"Enzyme-linked immunosorbent assay (ELISA)",
"HAB",
"Harmful Algal Bloom",
"Lake Anna",
"Maine",
"Massachusetts",
"Mattaponi River",
"Microcystin",
"New Jersey",
"New York",
"North Anna River",
"Pamunkey River",
"Peconic River",
"Peconic River Basin",
"Penobscot River",
"Penobscot River Basin",
"Pheophytin-a",
"Quantitative polymerase chain reaction (qPCR)",
"SPATT",
"Salem River",
"Salem River Basin",
"Santuit Pond",
"Santuit River",
"Santuit River Basin",
"Saxitoxin",
"Shinnecock Canal",
"Solid Phase Adsorption Toxin Tracking",
"USGS:6464e4d3d34ec179a83da4aa",
"Virginia",
"Water quality",
"York River",
"York River Basin",
"anaC gene",
"biota",
"cyanotoxin genetics",
"cyanotoxins",
"cyrA gene",
"environment",
"inlandWaters",
"mcyE gene",
"phytoplankton community composition",
"sxtA gene"
]
|
| modified | 2024-04-29T00:00:00Z |
| publisher |
{
"name": "U.S. Geological Survey",
"@type": "org:Organization"
}
|
| spatial | -83.1885, 36.5979, -66.9727, 47.3388 |
| theme |
[
"Geospatial"
]
|
| title | Cyanobacteria, Cyanotoxin, Cyanotoxin Synthetase Gene, and other Water-Quality Data Collected from Five River Basins in the North Atlantic Appalachian Region, August through September, 2020 |