Data from: An in vitro analysis of how lactose modifies the gut microbiota structure and function of adults in a donor-independent manner
The impact of lactose on the gut microbiota of healthy adults was examined, using a short-term, in vitro strategy where fecal samples harvested from 18 donors were cultured anaerobically with and without lactose. Donors represented 3 adult age groups. Data collected include: amplicon sequencing of the V1-V2 regions of the 16S rRNA gene (available in the NCBI Sequence Read Archive associated with BioProject PRJNA883645: https://www.ncbi.nlm.nih.gov/bioproject/PRJNA883645), RT-qPCR of Bifidobacterium 16S rRNA genes, and short-chain fatty acid concentrations.
Resources in this dataset:
Resource Title: lactose sample attributes
File Name: lactose_sample_metadata.csv
Resource Title: lactose SCFA, pH, and gas production data
File Name: lactose_SCFA_data.csv
Resource Title: Total bacterial and Bifidobacterium genus qPCR
File Name: lactose_qPCR_all_bacteria_and_bifido.csv
Complete Metadata
| @type | dcat:Dataset |
|---|---|
| accessLevel | public |
| bureauCode |
[
"005:18"
]
|
| contactPoint |
{
"fn": "Narrowe, Adrienne",
"hasEmail": "mailto:adrienne.narrowe@usda.gov"
}
|
| description | <p>The impact of lactose on the gut microbiota of healthy adults was examined, using a short-term, in vitro strategy where fecal samples harvested from 18 donors were cultured anaerobically with and without lactose. Donors represented 3 adult age groups. Data collected include: amplicon sequencing of the V1-V2 regions of the 16S rRNA gene (available in the NCBI Sequence Read Archive associated with BioProject PRJNA883645: <a href="https://www.ncbi.nlm.nih.gov/bioproject/PRJNA883645">https://www.ncbi.nlm.nih.gov/bioproject/PRJNA883645</a>), RT-qPCR of Bifidobacterium 16S rRNA genes, and short-chain fatty acid concentrations. </p> <p>Resources in this dataset:</p> <ul> <li> <p>Resource Title: lactose sample attributes File Name: lactose_sample_metadata.csv</p> </li> <li> <p>Resource Title: lactose SCFA, pH, and gas production data File Name: lactose_SCFA_data.csv</p> </li> <li> <p>Resource Title: Total bacterial and Bifidobacterium genus qPCR File Name: lactose_qPCR_all_bacteria_and_bifido.csv</p> </li> </ul> |
| distribution |
[
{
"@type": "dcat:Distribution",
"title": "lactose_sample_metadata.csv",
"format": "csv",
"mediaType": "text/plain",
"downloadURL": "https://ndownloader.figshare.com/files/43734918"
},
{
"@type": "dcat:Distribution",
"title": "lactose_SCFA_data.csv",
"format": "csv",
"mediaType": "text/plain",
"downloadURL": "https://ndownloader.figshare.com/files/43734921"
},
{
"@type": "dcat:Distribution",
"title": "lactose_qPCR_all_bacteria_and_bifido.csv",
"format": "csv",
"mediaType": "text/plain",
"downloadURL": "https://ndownloader.figshare.com/files/43734930"
}
]
|
| identifier | 10.15482/USDA.ADC/1529150 |
| keyword |
[
"16S rRNA sequence data",
"ARS",
"NP306",
"data.gov",
"lactose",
"short chain fatty acids"
]
|
| license | https://creativecommons.org/licenses/by/4.0/ |
| modified | 2025-11-21 |
| programCode |
[
"005:040"
]
|
| publisher |
{
"name": "Agricultural Research Service",
"@type": "org:Organization"
}
|
| spatial |
"{"type": "Point", "coordinates": [-75.187092456102, 40.078126249741]}"
|
| temporal | 2021-06-01/2021-06-01 |
| title | Data from: An in vitro analysis of how lactose modifies the gut microbiota structure and function of adults in a donor-independent manner |
