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Data from: Biological Soil Amendments Can Support Survival of Pathogenic and Non-Pathogenic Escherichia coli in Soils and Sporadic Transfer to Romaine Lettuce
Biological soil amendments are an essential input in organic lettuce production. However, these BSAs can introduce or transfer pathogens like Escherichia coli O157:H7 to lettuce in pre-harvest environments. This study evaluated the effect of BSAs on survival of non-pathogenic and pathogenic E. coli in soils and transfer to lettuce. Romaine lettuce was grown with controlled light, temperature, and relative humidity. Soil was amended (side-dressed) with either heat treated poultry pellets (HTPP), HTPP with corn steep liquor (CSL), seabird guano (SBG), SBG with CSL, or left unamended (UA). Soils were co-inoculated with non-pathogenic, rifampicin-resistant E. coli TVS 353 and two chloramphenicol-resistant E. coli O157:H7 isolates (100 mL of 106 CFU/mL). E. coli survival over 28 days was evaluated. On day 28, Romaine lettuce was harvested, and presence of E. coli was determined.
Resources
2 resources available
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Ecoli_levels_recovered_from_amended_soil.xlsx
XLSX -
Soil_Temperature&Moisture.xlsx
XLSX
Complete Metadata
| @type | dcat:Dataset |
|---|---|
| accessLevel | public |
| bureauCode |
[
"005:18"
]
|
| contactPoint |
{
"fn": "Sharma, Manan",
"hasEmail": "mailto:manan.sharma@usda.gov"
}
|
| description | <p>Biological soil amendments are an essential input in organic lettuce production. However, these BSAs can introduce or transfer pathogens like <em>Escherichia coli</em> O157:H7 to lettuce in pre-harvest environments. This study evaluated the effect of BSAs on survival of non-pathogenic and pathogenic <em>E. coli</em> in soils and transfer to lettuce. Romaine lettuce was grown with controlled light, temperature, and relative humidity. Soil was amended (side-dressed) with either heat treated poultry pellets (HTPP), HTPP with corn steep liquor (CSL), seabird guano (SBG), SBG with CSL, or left unamended (UA). Soils were co-inoculated with non-pathogenic, rifampicin-resistant <em>E. coli</em> TVS 353 and two chloramphenicol-resistant <em>E. coli</em> O157:H7 isolates (100 mL of 10<sup>6</sup> CFU/mL). <em>E. coli</em> survival over 28 days was evaluated. On day 28, Romaine lettuce was harvested, and presence of <em>E. coli</em> was determined.</p> |
| distribution |
[
{
"@type": "dcat:Distribution",
"title": "Ecoli_levels_recovered_from_amended_soil.xlsx",
"format": "xlsx",
"mediaType": "application/vnd.openxmlformats-officedocument.spreadsheetml.sheet",
"downloadURL": "https://ndownloader.figshare.com/files/49136773"
},
{
"@type": "dcat:Distribution",
"title": "Soil_Temperature&Moisture.xlsx",
"format": "xlsx",
"mediaType": "application/vnd.openxmlformats-officedocument.spreadsheetml.sheet",
"downloadURL": "https://ndownloader.figshare.com/files/49136776"
}
]
|
| identifier | 10.15482/USDA.ADC/26999374.v1 |
| keyword |
[
"Bacterial survival studies",
"E. coli O157: H7",
"biological soil amendments",
"fresh produce contamination",
"produce safety"
]
|
| license | https://creativecommons.org/licenses/by/4.0/ |
| modified | 2025-06-30 |
| programCode |
[
"005:040"
]
|
| publisher |
{
"name": "Agricultural Research Service",
"@type": "org:Organization"
}
|
| temporal | 2023-08-21/2023-09-18 |
| title | Data from: Biological Soil Amendments Can Support Survival of Pathogenic and Non-Pathogenic Escherichia coli in Soils and Sporadic Transfer to Romaine Lettuce |
