Data from: Detection and differentiation of low virulence and virulent Orthoavulavirus javaense using a molecular beacon with RT-LAMP
Resources
31 resources available
-
SR-suppl_material.zip
ZIP -
Figure 1 Alignment.fasta
FASTA -
Alignment-Class II complete genomes May 2022.fasta
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Figure 3 and Supplementary Figure 2 MB-LAMP Plate reader fluorescence 29Aug23.xlsx
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Figure 2 Plasmids Additional Replicate 1 11Aug23.eds
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Figure 3 IVT RNA LOD AgPath Fusion qRT-PCR 29Aug23.eds
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Figure 2 Plasmids Additional Replicate 2 11Aug23.eds
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Figure 2 Plasmids Additional Replicate 3 11Aug23.eds
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Figure 4 and Figure 5 IVT RNA plate reader data 01sep23.xlsx
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Figure 3 MB-LAMP extra 30 min 29Aug23.eds
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Figure 2 Plasmids Replicate 2 08Aug23.eds
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Figure 4 and Figure 5 IVT RNA plate reader values 06Sep23.xlsx
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Figure 2 Plasmids Replicate 3 08Aug23.eds
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Figure 2 Plasmids Replicate 1 22Jul23.eds
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Figure 3 MB-LAMP 29Aug23 first hour.eds
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Figure 5 AgPath Fusion Lentos 1ng per rxn 18Oct23.eds
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Figure 5 Agpath qRT-PCR IVT RNA 01Sep23.eds
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Figure 5 AgPath qRT-PCR IVT RNA 06Sep23.eds
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Figure 5 Fusion AgPath velos 1ng 20Oct23.eds
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Figure 4 Beacon specificity 01Sep23 and Figure 5 IVT RNAs.eds
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Figure 5 Matrix AgPath Lento screen 1ng 17Oct23.eds
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Figure 4 Beacon specificity 06Sep23 and Figure 5 IVT RNAs.eds
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Figure 5 Matrix AgPath Velos 1ng 20Oct23.eds
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Figure 5 18Oct23 MB-LAMP Lentos total RNA 1ng per rxn.eds
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Figure 5 Plate reader data Lentos 18Oct23.xlsx
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Figure 5 plate reader data Velos 20oct23.xlsx
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Figure 6 EID50 dilutions LaSota M gene AgPath 16Nov23.eds
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Figure 6 LaSota EID50 diluteions plate reader data 16Nov23.xlsx
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Figure 5 MB-LAMP Velos RNA screen 1ng 20Oct23.eds
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Figure 6 IVT RNA spiked OP and CL swabs MB-LAMP 17Nov23.eds
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Figure 6 MB-LAMP 16Nov23 EID50 LaSota rapid lysis.eds
EDS
Complete Metadata
| @type | dcat:Dataset |
|---|---|
| accessLevel | public |
| accrualPeriodicity | R/P1D |
| bureauCode |
[
"005:18"
]
|
| contactPoint |
{
"fn": "Bakre, Abhijeet A.",
"hasEmail": "mailto:abhijeet.bakre@usda.gov"
}
|
| description | <p>This dataset accompanies the manuscript <a href="https://www.nature.com/articles/s41598-024-68816-7" target="_blank">Detection and differentiation of low virulence and virulent Orthoavulavirus javaense using a molecular beacon with RT-LAMP | Scientific Reports (nature.com)</a> published on 08/05/2024. The Orthoavulavirus javaense also called as Newcastle Disease virus is the causative of Newcastle disease a significant problem for the poultry industry. Virulent strains of this virus can cause extensive mortality and economic loss to the poultry industry. Current diagnostic methods rely on polymerase chain reaction targeting the fusion gene of this virus and this method is not only expensive and time consuming, but is also not field applicable. The dataset reported here and the manuscript published describe an assay that can be applied to field diagnosis of NDV via a colorimetric and fluorometric assay. The MB-RT-LAMP assay enables rapid detection of viruses from field samples without the need for RNA isolation and also enable rapid differentiation of virulent vs low virulence viruses. The data provided here demonstrate the sequence analysis undertaken to develop the MB-RT-LAMP primers and probes, the supporting data show the conditions tested to identify optimal parameters.</p> |
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|
| identifier | 10.15482/USDA.ADC/26792542.v1 |
| keyword |
[
"Diagnostic assay",
"Loop-mediated isothermal amplification",
"Newcastle disease virus"
]
|
| license | https://creativecommons.org/licenses/by/4.0/ |
| modified | 2025-06-03 |
| programCode |
[
"005:040"
]
|
| publisher |
{
"name": "Agricultural Research Service",
"@type": "org:Organization"
}
|
| temporal | 2023-03-01/2024-08-05 |
| title | Data from: Detection and differentiation of low virulence and virulent Orthoavulavirus javaense using a molecular beacon with RT-LAMP |
