Data from: Discovery of MicroRNAs of the Stable Fly (Diptera: Muscidae) by High-Throughput Sequencing

This dataset reports discovery and initial comparative analysis of 88 presumptive microRNA (miRNA) sequences from the stable fly, obtained using high-throughput sequencing of small RNAs. The majority of stable fly miRNAs were 22-23 nucleotides (nt) in length. Many miRNAs were arthropod specific, and several mature miRNA sequences showed greater sequence identity to miRNAs from other blood-feeding dipterans such as mosquitoes rather than to Drosophilids. This initial step in characterizing the stable fly microRNAome provides a basis for further analyses of life stage-specific and tissue-specific expression to elucidate their functional roles in stable fly biology. The stable fly, Stomoxys calcitrans (L.), is a serious ectoparasite affecting animal production and health of both animals and humans. Stable fly control relies largely on chemical insecticides; however, the development of insecticide resistance as well as environmental considerations requires continued discovery research to develop novel control technologies. MicroRNAs are a class of short noncoding RNAs that have been shown to be important regulators of gene expression across a wide variety of organisms, and may provide an innovative approach with regard to development of safer more targeted control technologies. Resources in this dataset:Resource Title: Table 1. miRNAs identified in pooled life stages of the stable fly S. calcitrans and corresponding miRBase 18 entries. File Name: Web Page, url: https://academic.oup.com/view-large/91020692 miRNAs identified in pooled life stages of the stable fly S. calcitran. and corresponding miRBase 18 entries (≥10 reads, sorted by abundance).

                                            [
  "005:18"
]
                                        

                                            {
  "fn": "Temeyer, Kevin B.",
  "hasEmail": "mailto:kevin.temeyer@usda.gov"
}
                                        

                                            [
  {
    "@type": "dcat:Distribution",
    "title": "https://academic.oup.com/view-large/91020692",
    "mediaType": "text/html",
    "downloadURL": "https://academic.oup.com/view-large/91020692"
  }
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                                            [
  "ARS",
  "Diptera",
  "Muscidae",
  "NP104",
  "Stomoxys calcitrans",
  "cattle",
  "cattle pest",
  "data.gov",
  "ectoparasite",
  "insecticide resistance",
  "livestock pest",
  "miRNA",
  "microRNA",
  "noncoding RNA",
  "stable fly"
]
                                        

                                            [
  "005:040"
]
                                        

                                            {
  "name": "Agricultural Research Service",
  "@type": "org:Organization"
}