Data from: Exposure of dengue-1 virus to Aedes aegypti and sensitivity to insecticides

"PhD obj3 LD50 data submitted April 21 2025": applied to Table 1 of manuscript; used to calculate the lethal dose at which 25%, 50%, and 90% of the organisms (Aedes aegypti) die (LD25, LD50, LD90) using Probit software; PoloPlus - Leora Software. These data were generated by RLA under the supervision of KJL in July-August, 2021, at the USDA-ARS Center for Medical, Agricultural and Veterinary Entomology (USDA-ARS-CMAVE) facility in Gainesville, FL. using dilutions of technical malathion, technical permethrin, and rapeseed methyl ester. These values were generated in order to identify an LD50 dose to apply to 17d old female Ae. aegypti (7 days old prior to blood meal), 10 days after feeding on a blood meal containing dengue-1 virus. Ae. aegypti to determine the LD50 were blood fed at 7 d old, and aged 10 d after blood feeding, then exposed to the dilutions of technical malathion, technical permethrin, and rapeseed methyl ester."PhD Obj3 dengue mortality check submitted April 21 2025": used to populate the results for Table 2 and Table 3; describing the differences in mortality associated with exposure to a dengue-1 treated bloodmeal and LD50 pesticide/control treatment exposure in Aedes aegypti mosquitoes. Analysis was conducted using R. These data were generated by RLA under the supervision of BWA at the University of Florida - Florida Medical Entomology Laboratory (UF-FMEL) facility in Vero Beach, FL between September and November 2021. Ae. aegypti females were aged to 7 d, provided a dengue-1 tainted blood meal or sham blood meal, allowed to age for 10 d after blood feeding, then a topical LD50 dose of technical permethrin, technical malathion diluted in rapeseed methyl ester or neat rapeseed methyl ester as a control were applied and mortality was recorded at 24 h and 48 h. Following mortality checks, Ae. aegypti were frozen (-80 deg C) and checked for the presence of virus using RT-qPCR.

                                            [
  "005:18"
]
                                        

                                            {
  "fn": "Aldridge, Robert, L.",
  "hasEmail": "mailto:Robert.Aldridge@usda.gov"
}
                                        

                                            [
  {
    "@type": "dcat:Distribution",
    "title": "PhD obj3 LD50 data submitted April 21 2025.csv",
    "format": "csv",
    "mediaType": "text/csv",
    "downloadURL": "https://ndownloader.figshare.com/files/53863586"
  },
  {
    "@type": "dcat:Distribution",
    "title": "PhD Obj3 dengue mortality check submitted April 21 2025.csv",
    "format": "csv",
    "mediaType": "text/csv",
    "downloadURL": "https://ndownloader.figshare.com/files/53863589"
  }
]
                                        

                                            [
  "insecticide susceptibility bioassay",
  "malathion",
  "permethrin insecticide",
  "topical bioassay"
]
                                        

                                            [
  "005:040"
]
                                        

                                            {
  "name": "Agricultural Research Service",
  "@type": "org:Organization"
}