Data from: Full-length 16S rRNA sequencing on target microbe establishment in laboratory and mass-reared Mediterranean fruit fly
Study aim was to determine establishment success of antibiotic resistance bacteria in laboratory and mass-reared Mediterranean fruit fly (Ceratitis capitata). Experiments conducted in Hilo, HI, USA by the USDA ARS. Datasets include two different datatypes: 1) data from plate counts (CFUs) on nutrient-rich and antibiotic-amended data and 2) amplicon sequence variant (ASVs) count data from full-length 16S rRNA sequencing using PacBio Kinnex mas-Seq on a PacBio Revio sequencer and associated taxonomy and .fasta files. Experiments were performed with two different fly lines, one maintained by the USDA ARS and another by California Department of Agriculture (CDFA). Experiments were performed with a target Enterobacter strain that was transformed with mScarlet and antibiotic resistance selection markers. Experiments evaluated the impact of fly age and inoculation formulation (via diet) on the conditions in which bacteria would be established under different dietary conditions. Data show that liquid diets support establishment of bacteria, regardless of age class and source. Sequence data suggest additional Enterobacter strains are present in flies beyond the target strain and these can be distinguished via full-length 16S rRNA sequencing, but not by shorter amplicon fragments.
Complete Metadata
| @type | dcat:Dataset |
|---|---|
| accessLevel | public |
| bureauCode |
[
"005:18"
]
|
| contactPoint |
{
"fn": "Mason, Charles J.",
"hasEmail": "mailto:charles.mason@usda.gov"
}
|
| description | <p dir="ltr">Study aim was to determine establishment success of antibiotic resistance bacteria in laboratory and mass-reared Mediterranean fruit fly (<i>Ceratitis capitata</i>). Experiments conducted in Hilo, HI, USA by the USDA ARS. Datasets include two different datatypes: 1) data from plate counts (CFUs) on nutrient-rich and antibiotic-amended data and 2) amplicon sequence variant (ASVs) count data from full-length 16S rRNA sequencing using PacBio Kinnex mas-Seq on a PacBio Revio sequencer and associated taxonomy and .fasta files. Experiments were performed with two different fly lines, one maintained by the USDA ARS and another by California Department of Agriculture (CDFA). Experiments were performed with a target <i>Enterobacter </i>strain that was transformed with mScarlet and antibiotic resistance selection markers. Experiments evaluated the impact of fly age and inoculation formulation (via diet) on the conditions in which bacteria would be established under different dietary conditions. Data show that liquid diets support establishment of bacteria, regardless of age class and source. Sequence data suggest additional <i>Enterobacter </i>strains are present in flies beyond the target strain and these can be distinguished via full-length 16S rRNA sequencing, but not by shorter amplicon fragments.</p> |
| distribution |
[
{
"@type": "dcat:Distribution",
"title": "'24 Inoc USDA NAL Submisson.zip",
"format": "zip",
"mediaType": "application/zip",
"downloadURL": "https://ndownloader.figshare.com/files/52696796"
}
]
|
| identifier | 10.15482/USDA.ADC/28514825.v1 |
| keyword |
[
"16S rRNA",
"Enterobacter",
"Mediteranean fruit fly",
"Microbiome"
]
|
| license | https://creativecommons.org/publicdomain/zero/1.0/ |
| modified | 2025-09-24 |
| programCode |
[
"005:040"
]
|
| publisher |
{
"name": "Agricultural Research Service",
"@type": "org:Organization"
}
|
| temporal | 2024-01-01/2025-01-01 |
| title | Data from: Full-length 16S rRNA sequencing on target microbe establishment in laboratory and mass-reared Mediterranean fruit fly |
