Data from: Host usage in <i>Aedes aegypti</i> collected from Houston, Texas and Phoenix, Arizona using whole mosquito third-generation sequencing blood meal analysis
Aedes aegypti is the primary vector of several viruses of international public health concern, including Zika, dengue, yellow fever, and chikungunya. Their synanthropic ecology and establishment in tropical, sub-tropical, and temperate areas make Ae. aegypti one of the most medically relevant mosquito species in the world. While they have been reported to be highly anthropophilic, several studies indicate a broader host range. They are also reported to take multiple bloodmeals between gonotrophic cycles. This consumption of multiple bloodmeals makes determination of host usage difficult when using typical Sanger sequencing methods due to sequence overlap. In this study, we examined host usage of Ae. aegypti in Maricopa County, Arizona and Harris County, Texas, using a Nanopore-based third-generation sequencing protocol to alleviate this issue.This repository contains files related to the methods described in this study, sequencing output metadata files, and the database files and the bioinformatic process used for the analysis of the sequencing data.
Complete Metadata
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| description | <p dir="ltr"><i>Aedes aegypti</i> is the primary vector of several viruses of international public health concern, including Zika, dengue, yellow fever, and chikungunya. Their synanthropic ecology and establishment in tropical, sub-tropical, and temperate areas make <i>Ae. aegypti</i> one of the most medically relevant mosquito species in the world. While they have been reported to be highly anthropophilic, several studies indicate a broader host range. They are also reported to take multiple bloodmeals between gonotrophic cycles. This consumption of multiple bloodmeals makes determination of host usage difficult when using typical Sanger sequencing methods due to sequence overlap. In this study, we examined host usage of <i>Ae. aegypti </i>in Maricopa County, Arizona and Harris County, Texas, using a Nanopore-based third-generation sequencing protocol to alleviate this issue.</p><p dir="ltr">This repository contains files related to the methods described in this study, sequencing output metadata files, and the database files and the bioinformatic process used for the analysis of the sequencing data.</p> |
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|
| identifier | 10.15482/USDA.ADC/26018947.v1 |
| keyword |
[
"Aedes aegypti",
"Houston, Texas",
"Phoenix, Arizona",
"blood meal analysis",
"nanopore sequencing"
]
|
| license | https://creativecommons.org/publicdomain/zero/1.0/ |
| modified | 2025-05-19 |
| programCode |
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|
| publisher |
{
"name": "Agricultural Research Service",
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|
| temporal | 2019-06-01/2021-10-31 |
| title | Data from: Host usage in <i>Aedes aegypti</i> collected from Houston, Texas and Phoenix, Arizona using whole mosquito third-generation sequencing blood meal analysis |
