Data from: Net release and uptake of xenometabolites across intestinal, hepatic, muscle, and renal tissue beds in healthy conscious pigs

Data and supplemental figures for manuscript titled "Net release and uptake of xenometabolites across intestinal, hepatic, muscle, and renal tissue beds in healthy conscious pigs." We used a catheterized conscious pig model to estimate inter-organ flux of xenometabolites, derivatives, and bile acids using LC/MS. Female pigs (n=12; 2-3 months old; 25.6 ± 2.2 kg) had surgically-implanted catheters across portal-drained viscera (PDV), splanchnic area (SPL), liver, kidney, and hindquarter muscle. Overnight fasted arterial and venous plasma was collected simultaneously in a conscious state and stored at -80°C. Thawed samples were analyzed by liquid chromatography-mass spectrometry. Plasma flow was determined with para-aminohippuric acid dilution technology and used to calculate net organ balance for each metabolite. This repository contains the LC/MS data and supplemental figures for the submitted manuscript. Resources in this dataset:Resource Title: Organ Net Balance Data for Mercer et al.. File Name: Mercer et al LCMS XM Organ Flux Data.xlsxResource Description: Calculated net balances for LC/MS data from Mercer et al. Resource Title: LC/MS data for Mercer et al.. File Name: Mercer et al LCMS XM raw data.xlsxResource Description: LC/MS peak area data for xenometabolites in Mercer et al.Resource Title: Mercer et al Supplemental Figure 2. File Name: Mercer et al Fig S2.pdfResource Description: Supplemental Figure 2 for Mercer et al manuscript: Absolute differences of xenometabolites from catheter ports in conscious overnight-fasted female pigs (n=12). Sampling sites included arterial, venous, renal, portal, and hepatic placed catheters. Resource Title: Mercer et al Supplemental Figure 1. File Name: Mercer et al Fig S1.pdfResource Description: Supplemental Figure 1 for Mercer et al manuscript: Supplemental Figure 1: Inter-organ flux of all xenometablites in conscious overnight-fasted female pigs (n=12). A negative flux reflects net uptake (flux < 0; indicated by bottom arrow) by the organ, whereas a positive flux reflects a net release (flux > 0; indicated by top arrow) by the organ. Plasma flow measurements were determined with the para-aminohippuric acid dilution method, and were used along with metabolite concentrations to calculate flux (see Methods). R at top of arrow indicates ‘Release’, while U indicates ‘Uptake’. Resource Title: Supplemental Table 1 for Mercer et al.. File Name: Mercer et al Table S1.xlsxResource Description: Mercer et al Supplemental Table 1: Raw differences of identified xenometabolites from port samples in conscious adult female pigs (n=10-12).

                                            [
  "005:18"
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                                            {
  "fn": "Piccolo, Brian",
  "hasEmail": "mailto:bdpiccolo@uams.edu"
}
                                        

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                                            [
  "ARS",
  "NP107",
  "data.gov",
  "inter organ balance",
  "metabolomics",
  "pig model",
  "tissue flux",
  "xenometabolite"
]
                                        

                                            [
  "005:040"
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                                            {
  "name": "Agricultural Research Service",
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