Data from: Persistence of the Probiotic Lacticaseibacillus rhamnosus Strain GG (LGG) in an In Vitro Model of the Gut Microbiome
Using the SHIME (an in vitro simulator of the human gut microbiome) we tracked the fate of the probiotic Lacticaseibacillus rhamnosus GG (LGG) over time and across colonic regions. Using fecal inoculum from three healthy human donors, reactors were established representing three colonic regions and both the luminal and mucosal microbiome in those regions. Community composition before, during, and after inoculation of the reactors with LGG as well as short chain fatty acid concentrations representing microbiome metabolic outputs. This dataset includes short-chain fatty acid concentrations and qPCR-based cell concentrations. Raw 16S rRNA amplicon sequencing of the V1-V2 regions can be found in the NCBI Sequence Read Archive associated with BioProject PRJNA893635: https://www.ncbi.nlm.nih.gov/bioproject/PRJNA893635.
Resources in this dataset:
Resource Title: Short Chain Fatty Acid concentrations
File Name: LGG_SCFA_data.csv
Resource Title: LGG qPCR concentrations
File Name: LGG_qPCR_data.csv
Resource Title: Sample metadata
File Name: LGG_16S_metadata.csv
Complete Metadata
| @type | dcat:Dataset |
|---|---|
| accessLevel | public |
| bureauCode |
[
"005:18"
]
|
| contactPoint |
{
"fn": "Narrowe, Adrienne",
"hasEmail": "mailto:adrienne.narrowe@usda.gov"
}
|
| description | <p>Using the SHIME (an in vitro simulator of the human gut microbiome) we tracked the fate of the probiotic Lacticaseibacillus rhamnosus GG (LGG) over time and across colonic regions. Using fecal inoculum from three healthy human donors, reactors were established representing three colonic regions and both the luminal and mucosal microbiome in those regions. Community composition before, during, and after inoculation of the reactors with LGG as well as short chain fatty acid concentrations representing microbiome metabolic outputs. This dataset includes short-chain fatty acid concentrations and qPCR-based cell concentrations. Raw 16S rRNA amplicon sequencing of the V1-V2 regions can be found in the NCBI Sequence Read Archive associated with BioProject PRJNA893635: <a href="https://www.ncbi.nlm.nih.gov/bioproject/PRJNA893635">https://www.ncbi.nlm.nih.gov/bioproject/PRJNA893635</a>.</p> <p>Resources in this dataset:</p> <ul> <li> <p>Resource Title: Short Chain Fatty Acid concentrations File Name: LGG_SCFA_data.csv</p> </li> <li> <p>Resource Title: LGG qPCR concentrations File Name: LGG_qPCR_data.csv</p> </li> <li> <p>Resource Title: Sample metadata File Name: LGG_16S_metadata.csv</p> </li> </ul> |
| distribution |
[
{
"@type": "dcat:Distribution",
"title": "LGG_16S_metadata.csv",
"format": "csv",
"mediaType": "application/csv",
"downloadURL": "https://ndownloader.figshare.com/files/44540666"
},
{
"@type": "dcat:Distribution",
"title": "LGG_SCFA_data.csv",
"format": "csv",
"mediaType": "application/csv",
"downloadURL": "https://ndownloader.figshare.com/files/44540672"
},
{
"@type": "dcat:Distribution",
"title": "LGG_qPCR_data.csv",
"format": "csv",
"mediaType": "application/csv",
"downloadURL": "https://ndownloader.figshare.com/files/44540675"
}
]
|
| identifier | 10.15482/USDA.ADC/1529138 |
| keyword |
[
"16S rRNA sequence data",
"ARS",
"NP306",
"data.gov",
"probiotics",
"short chain fatty acids"
]
|
| license | https://creativecommons.org/licenses/by/4.0/ |
| modified | 2025-11-21 |
| programCode |
[
"005:040"
]
|
| publisher |
{
"name": "Agricultural Research Service",
"@type": "org:Organization"
}
|
| spatial |
"{"type": "Point", "coordinates": [-75.187092456102, 40.078126249741]}"
|
| temporal | 2018-10-16/2019-04-07 |
| title | Data from: Persistence of the Probiotic Lacticaseibacillus rhamnosus Strain GG (LGG) in an In Vitro Model of the Gut Microbiome |
