Decontamination of B. globigii spores from drinking water infrastructure using disinfectants

Decontamination of Bacillus spores adhered to common drinking water infrastructure surfaces was evaluated using a variety of disinfectants. Corroded iron and cement-mortar lined iron represented the infrastructure surfaces, and were conditioned in a 23 m long, 15 cm diameter (75 ft long, 6 in diameter) pilot-scale drinking water distribution pipe system. Decontamination was evaluated using increased water velocity (flushing) alone at 0.5 m sec-1 (1.7 ft sec-1), as well as free chlorine (5 and 25 mg L-1), monochloramine (25 mg L-1), chlorine dioxide (5 and 25 mg L-1), ozone (2.0 mg L-1), peracetic acid 25 mg L-1) and acidified nitrite (0.1 mol L-1 at pH 2 and 3), all followed by flushing at 0.3 m sec-1 (1 ft sec-1). Flushing alone reduced the adhered spores by 0.5 and 2.0 log10 from iron and cement-mortar, respectively. Log10 reduction on corroded iron pipe wall coupons ranged from 1.0 to 2.9 at respective chlorine dioxide concentrations of 5 and 25 mg L-1, although spores were undetectable on the iron surface during disinfection at 25 mg L-1. Acidified nitrite (pH 2, 0.1 mol L-1) yielded no detectable spores on the iron surface during the flushing phase after disinfection. Chlorine dioxide was the best performing disinfectant with >3.0 log10 removal from cement-mortar at 5 and 25 mg L-1.The data show that free chlorine, monochloramine, ozone and chlorine dioxide followed by flushing can reduce adhered spores by >3.0 log10 on cement-mortar. This dataset is associated with the following publication: Szabo , J., G. Meiners, L. Heckman, G. Rice , and J. Hall. Decontamination of Bacillus spores adhered to iron and cement-mortar drinking water infrastructure in a model system using disinfectants. JOURNAL OF ENVIRONMENTAL MANAGEMENT. Elsevier Science Ltd, New York, NY, USA, 187: 1-7, (2017).

                                            [
  "020:00"
]
                                        

                                            {
  "fn": "Jeffrey Szabo",
  "hasEmail": "mailto:szabo.jeff@epa.gov"
}
                                        

                                            [
  {
    "title": "Disinfection Loop Data Analysis 11252016.xlsx",
    "mediaType": "application/vnd.openxmlformats-officedocument.spreadsheetml.sheet",
    "downloadURL": "https://pasteur.epa.gov/uploads/453/Disinfection%20Loop%20Data%20Analysis%2011252016.xlsx"
  }
]
                                        

                                            [
  "Bacillus anthracis",
  "Decontamination",
  "Ozone",
  "acidified nitrite",
  "bacillus atrophaeus",
  "bacterial spores",
  "cement-mortar",
  "chlorine dioixde",
  "drinking water",
  "flushing",
  "free chlorine",
  "infrastructure",
  "iron",
  "monochloramine",
  "peracetic acid",
  "water pipes"
]
                                        

                                            [
  "020:060"
]
                                        

                                            {
  "name": "U.S. EPA Office of Research and Development (ORD)",
  "subOrganizationOf": {
    "name": "U.S. Environmental Protection Agency",
    "subOrganizationOf": {
      "name": "U.S. Government"
    }
  }
}
                                        

                                            [
  "https://doi.org/10.1016/j.jenvman.2016.11.024"
]
                                        

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