Derived ELISA Values for Hawaiian Island forest bird avian malaria detection using whole blood preserved in lysis buffer
It is unclear whether DNA lysis buffers used for preservation of whole blood samples from Hawaiian forest birds cause denaturation and loss of antigen binding capability of antibody molecules. If their antigen binding capability is not affected, then the samples can be used in serological assays to provide an independent assessment of the accuracy of PCR (polymerase chain reaction) diagnostic tests for avian malaria.
This data set provides derived Percent ELISA (Enzyme linked Immunosorbant Assay) Values calculated from adjusted absorbance measurements that were collected with a BioRad Model 3550 ELISA plate reader at a wavelength setting of 405 nm.
Complete Metadata
| accessLevel | public |
|---|---|
| bureauCode |
[
"010:12"
]
|
| contactPoint |
{
"fn": "Carter T Atkinson",
"@type": "vcard:Contact",
"hasEmail": "mailto:catkinson@usgs.gov"
}
|
| description | It is unclear whether DNA lysis buffers used for preservation of whole blood samples from Hawaiian forest birds cause denaturation and loss of antigen binding capability of antibody molecules. If their antigen binding capability is not affected, then the samples can be used in serological assays to provide an independent assessment of the accuracy of PCR (polymerase chain reaction) diagnostic tests for avian malaria. This data set provides derived Percent ELISA (Enzyme linked Immunosorbant Assay) Values calculated from adjusted absorbance measurements that were collected with a BioRad Model 3550 ELISA plate reader at a wavelength setting of 405 nm. |
| distribution |
[
{
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"title": "Digital Data",
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"accessURL": "https://doi.org/10.5066/P98CVJJG",
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"title": "Original Metadata",
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|
| identifier | http://datainventory.doi.gov/id/dataset/USGS_5e98e22b82ce172707f6f981 |
| keyword |
[
"Chlorodrepanis virens",
"ELISA",
"Hakalau Forest National Wildlife Refuge",
"Hawaii",
"Hawaii Amakihi",
"Hawaiian Islands",
"Lysis Buffer",
"PCR",
"Plasmodium relictum",
"USGS:5e98e22b82ce172707f6f981",
"Western blot",
"avian malaria",
"biota",
"enzyme linked immunosorbant assay",
"immunoblot",
"polymerase chain reaction"
]
|
| modified | 2020-08-27T00:00:00Z |
| publisher |
{
"name": "U.S. Geological Survey",
"@type": "org:Organization"
}
|
| spatial | -156.2640, 18.8023, -154.6820, 20.3395 |
| theme |
[
"Geospatial"
]
|
| title | Derived ELISA Values for Hawaiian Island forest bird avian malaria detection using whole blood preserved in lysis buffer |
