Detection of the Aquatic Invasive Species (AIS) Elodea canadensis and E. nuttallii Using Environmental DNA (eDNA), Alaska
This data set is comprised of one table with sampling information and National Center for Biotechnology Information (NCBI) BioProject accession numbers for sequence information of this amplicon-based study targeting Elodea canadensis and E. nuttallii in freshwater systems of Alaska from environmental samples. Highly conserved primers which can differentiate these species of interest were developed for four portions of Elodea mtDNA genes (ITS1-5.8S, atpB-rbcL, and two variations of trnL-trnF). The reference sequences and conserved primer sets to identify species present were developed using publicly available data from NCBI GenBank (https://www.ncbi.nlm.nih.gov/genbank/).
Complete Metadata
| accessLevel | public |
|---|---|
| bureauCode |
[
"010:12"
]
|
| contactPoint |
{
"fn": "U.S. Geological Survey, Alaska Science Center",
"@type": "vcard:Contact",
"hasEmail": "mailto:gs-ak_asc_datamanagers@usgs.gov"
}
|
| description | This data set is comprised of one table with sampling information and National Center for Biotechnology Information (NCBI) BioProject accession numbers for sequence information of this amplicon-based study targeting Elodea canadensis and E. nuttallii in freshwater systems of Alaska from environmental samples. Highly conserved primers which can differentiate these species of interest were developed for four portions of Elodea mtDNA genes (ITS1-5.8S, atpB-rbcL, and two variations of trnL-trnF). The reference sequences and conserved primer sets to identify species present were developed using publicly available data from NCBI GenBank (https://www.ncbi.nlm.nih.gov/genbank/). |
| distribution |
[
{
"@type": "dcat:Distribution",
"title": "Digital Data",
"format": "XML",
"accessURL": "https://doi.org/10.5066/P94ZAUGK",
"mediaType": "application/http",
"description": "Landing page for access to the data"
},
{
"@type": "dcat:Distribution",
"title": "Original Metadata",
"format": "XML",
"mediaType": "text/xml",
"description": "The metadata original format",
"downloadURL": "https://data.usgs.gov/datacatalog/metadata/USGS.663d2da7d34eaf9729f78cc9.xml"
}
]
|
| identifier | http://datainventory.doi.gov/id/dataset/USGS_663d2da7d34eaf9729f78cc9 |
| keyword |
[
"5.8S",
"Aquatic ecosystems",
"Aquatic invasive species",
"Biota",
"Coastal ecosystems",
"DNA Primers",
"DNA sequencing",
"Elodea",
"Environment",
"Environmental DNA",
"Genetic markers",
"Genetics",
"Marine environment monitoring",
"Metabarcoding",
"Mitochondrial DNA",
"Polymerase chain reaction",
"Tundra ecosystems",
"USGS:663d2da7d34eaf9729f78cc9",
"Weeds, noxious plants, or invasive plants",
"Wetland ecosystems",
"Wildlife",
"atpB-rbcL",
"eDNA",
"trnL-trnF"
]
|
| modified | 2024-05-17T00:00:00Z |
| publisher |
{
"name": "U.S. Geological Survey",
"@type": "org:Organization"
}
|
| spatial | -179.8242, 50.2893, -128.6718, 71.8562 |
| theme |
[
"Geospatial"
]
|
| title | Detection of the Aquatic Invasive Species (AIS) Elodea canadensis and E. nuttallii Using Environmental DNA (eDNA), Alaska |
