Evaluation of 147 Perfluoroalkyl Substances for Immunosuppressive and other Activities Through Phenotypic Screening of Human Primary Cells

Dataset for journal article 'Evaluation of 147 Perfluoroalkyl Substances for Immunosuppressive and other Activities Through Phenotypic Screening of Human Primary Cells'. Per- and polyfluoroalkyl substances (PFAS) are a large class of chemicals in widespread use for diverse applications in commerce resulting in significant presence in the environment. Extensive studies of several of the highly produced members of the class have demonstrated potential for adverse health consequences to humans and the environment as well as highly pervasive and persistent exposures. Here we report testing results of 147 PFAS substances in a phenotypic screening platform of primary human cell co-culture systems, the BioMAP® Diversity PLUS panel, used to model complex tissue and disease biology of organs (vasculature, immune system, skin, lung) and general tissue biology. PFAS were tested at four concentrations ranging from approximately 0.06 to 60 micromolar in order to minimize influence from confounding effects of polypharmacology resulting from qualitatively different activities at higher concentrations. We also compared response profiles for all PFAS with an existing database of responses for the BioMAP assays to identify other potential mechanisms of activity for this diverse chemical family. This dataset is associated with the following publication: Houck, K., K. Friedman, M. Feshuk, G. Patlewicz, M. Smeltz, M. Clifton, B. Wetmore, S. Velichko, A. Berenyi, and E. Berg. Evaluation of 147 perfluoroalkyl substances for immunotoxic and other (patho)physiological activities through phenotypic screening of human primary cells. ALTEX. Society ALTEX Edition, Kuesnacht, SWITZERLAND, 40(2): 248-270, (2023).

                                            [
  "020:00"
]
                                        

                                            {
  "fn": "Katie Friedman",
  "hasEmail": "mailto:paul-friedman.katie@epa.gov"
}
                                        

                                            [
  {
    "title": "https://clowder.edap-cluster.com/datasets/61147fefe4b0856fdc65639b#folderId=61ae8137e4b0eee4f6b365f2",
    "accessURL": "https://clowder.edap-cluster.com/datasets/61147fefe4b0856fdc65639b#folderId=61ae8137e4b0eee4f6b365f2"
  }
]
                                        

                                            [
  "PFAS",
  "bioactivity screening",
  "chemical safety",
  "computational toxicology",
  "immunosuppression",
  "primary cells",
  "proteosome"
]
                                        

                                            [
  "020:000"
]
                                        

                                            {
  "name": "U.S. EPA Office of Research and Development (ORD)",
  "subOrganizationOf": {
    "name": "U.S. Environmental Protection Agency",
    "subOrganizationOf": {
      "name": "U.S. Government"
    }
  }
}
                                        

                                            [
  "https://doi.org/10.14573/altex.2203041"
]
                                        

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