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Evaluation of Cleaning and Disinfection Protocols for Commercial Farm Equipment Following a Foreign Animal Disease Outbreak

Published by U.S. EPA Office of Research and Development (ORD) | U.S. Environmental Protection Agency | Metadata Last Checked: August 02, 2025 | Last Modified: 2021-08-02
Aims: Evaluate the microbiocidal efficacy of a cleaning and disinfection (C&D) treatment using stainless steel coupons applied to three common types of animal mortality transport vehicles when exposed to agricultural conditions. Methods: Metal test coupons, inoculated with bacteriophage MS2, were affixed to the undercarriage of three types of animal mortality transport vehicles at various locations. Coupons were grimed by maneuvering the test vehicles down a series of wet dirt roads. Coupons were attached and extracted at various points to evaluate C&D performance with and without grime. C&D efficacy using a water-supplied pressure washing system and a dilute sodium hypochlorite (NaOCl) solution was determined by comparing the difference in recovered viable virus between positive control coupons and test coupons. This dataset is associated with the following publication: Boe, T., W. Calfee, P. Lemieux, S. Serre, A. Abdel-Hady, M. Monge, D. Aslett, B. Akers, and J. Howard. Evaluation of Cleaning and Disinfection Protocols for Commercial Farm Equipment Following a Foreign Animal Disease Outbreak. Remediation Journal. John Wiley & Sons, Inc., Hoboken, NJ, USA, 33(4): 379-387, (2023).

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