Exposure of zebrafish larvae to relative microgravity between 5-6 days post-fertilization
The general objective of the study was to determine modulation of gene expression by environmental factors with a special emphasis on bone formation. For this reason the specific period of treatment was chosen between 5-6 days post-fertilization (dpf) when bone formation and calcification are taking place. This experiment was designed as a new type of gravitational experiment which we like to call relative microgravity referring to the fact that the larvae first grow in hyper gravity for 5 days and are then returned to 1g normal gravity for 1 day. Zebrafish embryos were placed on a Large Diameter Centrifuge at 3 hpf brought to a gravitational force of 3 g until 5 dpf. Reference embryos were kept in parallel at 1g (Inc). At 5dpf one batch was left at 3g (3g) one batch was returned to 1g (3g>1g) while a third batch was returned to 1g but left on the axis of the centrifuge (Axe; 3g>Axe). The experiment was repeated 4 times each time with 4 batches of 60 larvae.
Complete Metadata
| @type | dcat:Dataset |
|---|---|
| accessLevel | public |
| accrualPeriodicity | irregular |
| bureauCode |
[
"026:00"
]
|
| contactPoint |
{
"fn": "GeneLab Outreach",
"@type": "vcard:Contact",
"hasEmail": "mailto:genelab-outreach@lists.nasa.gov"
}
|
| description | The general objective of the study was to determine modulation of gene expression by environmental factors with a special emphasis on bone formation. For this reason the specific period of treatment was chosen between 5-6 days post-fertilization (dpf) when bone formation and calcification are taking place. This experiment was designed as a new type of gravitational experiment which we like to call relative microgravity referring to the fact that the larvae first grow in hyper gravity for 5 days and are then returned to 1g normal gravity for 1 day. Zebrafish embryos were placed on a Large Diameter Centrifuge at 3 hpf brought to a gravitational force of 3 g until 5 dpf. Reference embryos were kept in parallel at 1g (Inc). At 5dpf one batch was left at 3g (3g) one batch was returned to 1g (3g>1g) while a third batch was returned to 1g but left on the axis of the centrifuge (Axe; 3g>Axe). The experiment was repeated 4 times each time with 4 batches of 60 larvae. |
| distribution |
[
{
"@type": "dcat:Distribution",
"title": "Exposure of zebrafish larvae to relative microgravity between 5-6 days post-fertilization",
"format": "HTML",
"mediaType": "text/html",
"description": "GeneLab Study Page",
"downloadURL": "https://genelab-data.ndc.nasa.gov/genelab/accession/GLDS-60"
}
]
|
| identifier | nasa_genelab_GLDS-60_kdpk-5n4g |
| issued | 2021-05-21 |
| keyword |
[
"array-scanning-and-feature-extraction-protocol",
"growth-protocol",
"hypergravity",
"normalization-data-transformation-protocol",
"nucleic-acid-extraction-protocol",
"nucleic-acid-hybridization-to-array-protocol",
"nucleic-acid-labeling-protocol",
"p-mtab-43163",
"p-mtab-43164",
"p-mtab-43165",
"p-mtab-43166",
"p-mtab-43167",
"p-mtab-43168",
"p-mtab-43169",
"treatment-protocol"
]
|
| landingPage | https://data.nasa.gov/dataset/exposure-of-zebrafish-larvae-to-relative-microgravity-between-5-6-days-post-fertilization |
| license | http://www.usa.gov/publicdomain/label/1.0/ |
| modified | 2025-04-23 |
| programCode |
[
"026:005"
]
|
| publisher |
{
"name": "National Aeronautics and Space Administration",
"@type": "org:Organization"
}
|
| theme |
[
"Earth Science"
]
|
| title | Exposure of zebrafish larvae to relative microgravity between 5-6 days post-fertilization |
