Genetic linkage map and comparative genome analysis for the estuarine Atlantic killifish (Fundulus heteroclitus)

Genetic linkage maps are valuable tools in evolutionary biology; however, their availability for wild populations is extremely limited. Fundulus heteroclitus (Atlantic killifish) is a non-migratory estuarine fish that exhibits high allelic and phenotypic diversity partitioned among subpopulations that reside in disparate environmental conditions. An ideal candidate model organism for studying gene-environment interactions, the molecular toolbox for F. heteroclitus is limited. We identified hundreds of novel microsatellites which, when combined with existing microsatellites and single nucleotide polymorphisms (SNPs), were used to construct the first genetic linkage map for this species. By integrating independent linkage maps from three genetic crosses, we developed a consensus map containing 24 linkage groups, consistent with the number of chromosomes reported for this species. These linkage groups span 2300 centimorgans (cM) of recombinant genomic space, intermediate in size relative to the current linkage maps for the teleosts, medaka and zebrafish. Comparisons between fish genomes support a high degree of synteny between the consensus F. heteroclitus linkage map and the medaka and (to a lesser extent) zebrafish physical genome assemblies. This dataset is associated with the following publication: Waits , E., J. Martinson , B. Rinner, S. Morris, D. Proestou, D. Champlin , and D. Nacci. Genetic linkage map and comparative genome analysis for the estuarine Atlantic killifish (Fundulus heteroclitus). Open Journal of Genetics. Scientific Research Publishing, Inc., Irvine, CA, USA, 6: 28-38, (2016).

                                            [
  "020:00"
]
                                        

                                            {
  "fn": "Eric Waits",
  "hasEmail": "mailto:waits.eric@epa.gov"
}
                                        

                                            [
  {
    "title": "Waits et al Data.xlsx",
    "mediaType": "application/vnd.openxmlformats-officedocument.spreadsheetml.sheet",
    "downloadURL": "https://pasteur.epa.gov/uploads/237/Waits%20et%20al%20Data.xlsx"
  }
]
                                        

                                            [
  "Fundulus heteroclitus",
  "Genetic Linkage Map",
  "Microsatellite",
  "Single Nucleotide Polymorphism (SNP)",
  "Synteny Analysis"
]
                                        

                                            [
  "020:000"
]
                                        

                                            {
  "name": "U.S. EPA Office of Research and Development (ORD)",
  "subOrganizationOf": {
    "name": "U.S. Environmental Protection Agency",
    "subOrganizationOf": {
      "name": "U.S. Government"
    }
  }
}
                                        

                                            [
  "https://doi.org/10.4236/ojgen.2016.61004"
]
                                        

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