Genome sequencing of Curculio caryae adult female principal pseudohaplotype

The pecan weevil, Curculio caryae (Horn), is an obligate feeder of pecan and native hickory throughout North America. Subsequently it is a significant agricultural pest in commercial pecan orchards. In this study, we present a reference quality genome using deep-coverage, ~50x PacBio HiFi genome sequence reads, and chromatic confirmation, Hi-C, scaffolding. The final genome assembly is approximately 2.1 Gb, which was confirmed with flow cytometry. The primary genome scaffolds have an N50 of 132 Mb and a BUSCO completeness of 99.1% [S:97.5%, D:1.6%]. Furthermore, we employed PacBio long-read RNA, Iso-seq, for de novo annotation, followed by InterProscan to identify approximately 16,000 protein coding genes. Repeat content is extensive, contributing to >75% of the total genome. This data set provides a valuable resource for use in comparative genomics and evolutionary studies for an economically impactful group of insect pests that currently lack genomic resources.

                                            [
  "005:18"
]
                                        

                                            {
  "fn": "Perkin, Lindsey, C.",
  "hasEmail": "mailto:lindsey.perkin@usda.gov"
}
                                        

                                            [
  {
    "@type": "dcat:Distribution",
    "title": "https://www.ncbi.nlm.nih.gov/bioproject/?term=PRJNA813156",
    "mediaType": "text/html",
    "downloadURL": "https://www.ncbi.nlm.nih.gov/bioproject/?term=PRJNA813156"
  }
]
                                        

                                            [
  "Ag100Pest",
  "Curculio caryae",
  "HiFi genome assembly",
  "pecan weevil"
]
                                        

                                            [
  "005:040"
]
                                        

                                            {
  "name": "Agricultural Research Service",
  "@type": "org:Organization"
}