High-volume, label-free imaging for quantifying single-cell dynamics in iPSC colonies
This collection is comprised of multiple sets of experiments involving iPSCs with varying dosages of fluorescent light excitation. Samples were imaged every 2 minutes using phase-contrast microscopy for 20+ hours with or without fluorescence excitation. Each experiment was done on different days (i.e. exp0 vs exp1) but with triplicate wells (i.e. exp1-0, exp1-1, exp1-2). Additionally, data used to train our 2D U-Net and 3D U-Net models are included. Detailed information for each dataset can be found in Dataset_Information.xlsx.
Complete Metadata
| @type | dcat:Dataset |
|---|---|
| accessLevel | public |
| accrualPeriodicity | irregular |
| bureauCode |
[
"006:55"
]
|
| contactPoint |
{
"fn": "Anne L. Plant",
"hasEmail": "mailto:anne.plant@nist.gov"
}
|
| description | This collection is comprised of multiple sets of experiments involving iPSCs with varying dosages of fluorescent light excitation. Samples were imaged every 2 minutes using phase-contrast microscopy for 20+ hours with or without fluorescence excitation. Each experiment was done on different days (i.e. exp0 vs exp1) but with triplicate wells (i.e. exp1-0, exp1-1, exp1-2). Additionally, data used to train our 2D U-Net and 3D U-Net models are included. Detailed information for each dataset can be found in Dataset_Information.xlsx. |
| distribution |
[
{
"title": "WSDOM iPSC Tracking GitHub Page",
"format": "GitHub Python Files",
"accessURL": "https://github.com/usnistgov/WSDOM",
"description": "GitHub page with all scripts and models used for the image dataset."
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]
|
| identifier | ark:/88434/mds2-2960 |
| issued | 2023-10-02 |
| keyword |
[
"AI",
"U-Net",
"genetic engineering; regenerative medicine",
"pluripotent cell lines; quantitative live cell imaging; fluorescence microscopy; image data analysis; CNN"
]
|
| landingPage | https://data.nist.gov/od/id/mds2-2960 |
| language |
[
"en"
]
|
| license | https://www.nist.gov/open/license |
| modified | 2023-03-17 00:00:00 |
| programCode |
[
"006:045"
]
|
| publisher |
{
"name": "National Institute of Standards and Technology",
"@type": "org:Organization"
}
|
| theme |
[
"Bioscience:Cell biology"
]
|
| title | High-volume, label-free imaging for quantifying single-cell dynamics in iPSC colonies |
