In Vitro Metabolism of Imidacloprid and Acetamiprid in Rainbow Trout and Rat - Kolanczyk_A-dv4j_SDMP_20190918
The current study constitutes the first report of the metabolism of imidacloprid and acetamiprid in rainbow trout. Formation of respective metabolites 5-hydroxy-imidacloprid and N-desmethyl-acetamiprid was validated by analytical methods and demonstrated to be conserved across orders of biological organization in both microsomal and liver slice assays. Michaelis-Menten kinetics were determined for the microsomal conversion of imidacloprid to 5-hydroxy-imidacloprid in rainbow trout (Km=79.2 µM; Vmax=0.75 pmole/min/mg) and rat (Km=158.7 µM; Vmax=38.4 pmole/min/mg). Kinetics for the microsomal demethylation of acetamiprid to N-desmethyl-acetamiprid were determined in the rat (Km=70.9 µM; Vmax=4.10 pmoles/min/mg). N-desmethyl-acetamiprid was found in detectable but below quantifiable levels across the range of test concentrations which precluded a calculation of kinetic rate constants in rainbow trout (RBT). Ultimately, the formation of the metabolites 5-hydroxy-imidacloprid and N-desmethyl-acetamiprid was conserved across RBT and rat species.
This dataset is associated with the following publication:
Kolanczyk, R., M. Tapper, B. Sheedy, and J. Serrano. In vitro metabolism of imidacloprid and acetamiprid in rainbow trout and rat. XENOBIOTICA. Taylor & Francis, Inc., Philadelphia, PA, USA, 50(7): 805-814, (2020).
Complete Metadata
| accessLevel | public |
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| contactPoint |
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"fn": "Richard Kolanczyk",
"hasEmail": "mailto:kolanczyk.rick@epa.gov"
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| description | The current study constitutes the first report of the metabolism of imidacloprid and acetamiprid in rainbow trout. Formation of respective metabolites 5-hydroxy-imidacloprid and N-desmethyl-acetamiprid was validated by analytical methods and demonstrated to be conserved across orders of biological organization in both microsomal and liver slice assays. Michaelis-Menten kinetics were determined for the microsomal conversion of imidacloprid to 5-hydroxy-imidacloprid in rainbow trout (Km=79.2 µM; Vmax=0.75 pmole/min/mg) and rat (Km=158.7 µM; Vmax=38.4 pmole/min/mg). Kinetics for the microsomal demethylation of acetamiprid to N-desmethyl-acetamiprid were determined in the rat (Km=70.9 µM; Vmax=4.10 pmoles/min/mg). N-desmethyl-acetamiprid was found in detectable but below quantifiable levels across the range of test concentrations which precluded a calculation of kinetic rate constants in rainbow trout (RBT). Ultimately, the formation of the metabolites 5-hydroxy-imidacloprid and N-desmethyl-acetamiprid was conserved across RBT and rat species. This dataset is associated with the following publication: Kolanczyk, R., M. Tapper, B. Sheedy, and J. Serrano. In vitro metabolism of imidacloprid and acetamiprid in rainbow trout and rat. XENOBIOTICA. Taylor & Francis, Inc., Philadelphia, PA, USA, 50(7): 805-814, (2020). |
| distribution |
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"title": "Imidacloprid acetamiprid trout vs rat data.xlsx",
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"downloadURL": "https://pasteur.epa.gov/uploads/10.23719/1504449/Imidacloprid%20acetamiprid%20trout%20vs%20rat%20data.xlsx"
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| identifier | https://doi.org/10.23719/1504449 |
| keyword |
[
"Metabolism",
"Rat",
"acetamiprid",
"imidacloprid",
"in vitro",
"liver slice",
"microsomes",
"neonicotinoid",
"rainbow trout"
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| license | https://pasteur.epa.gov/license/sciencehub-license.html |
| modified | 2019-05-15 |
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| publisher |
{
"name": "U.S. EPA Office of Research and Development (ORD)",
"subOrganizationOf": {
"name": "U.S. Environmental Protection Agency",
"subOrganizationOf": {
"name": "U.S. Government"
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| references |
[
"https://doi.org/10.1080/00498254.2019.1694197"
]
|
| rights |
null
|
| title | In Vitro Metabolism of Imidacloprid and Acetamiprid in Rainbow Trout and Rat - Kolanczyk_A-dv4j_SDMP_20190918 |
