Kinesin-like protein CENP-E is upregulated in rheumatoid synovial fibroblasts
Our aim was to identify specifically expressed genes using RNA arbitrarily primed (RAP)-polymerase chain reaction (PCR) for differential display in patients with rheumatoid arthritis (RA). In RA, amplification of a distinct PCR product suitable for sequencing could be observed. Sequence analysis identified the PCR product as highly homologous to a 434 base pair segment of the human centromere kinesin-like protein CENP-E. Differential expression of CENP-E was confirmed by quantitative reverse transcription PCR, immunohistochemistry and in situ hybridization. CENP-E expression was independent from prednisolone and could not be completely inhibited by serum starvation. RAP-PCR is a suitable method to identify differentially expressed genes in rheumatoid synovial fibroblasts. Also, because motifs of CENP-E show homologies to jun and fos oncogene products and are involved in virus assembly, CENP-E may be involved in the pathophysiology of RA.
Complete Metadata
| @type | dcat:Dataset |
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[
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|
| description | Our aim was to identify specifically expressed genes using RNA arbitrarily primed (RAP)-polymerase chain reaction (PCR) for differential display in patients with rheumatoid arthritis (RA). In RA, amplification of a distinct PCR product suitable for sequencing could be observed. Sequence analysis identified the PCR product as highly homologous to a 434 base pair segment of the human centromere kinesin-like protein CENP-E. Differential expression of CENP-E was confirmed by quantitative reverse transcription PCR, immunohistochemistry and in situ hybridization. CENP-E expression was independent from prednisolone and could not be completely inhibited by serum starvation. RAP-PCR is a suitable method to identify differentially expressed genes in rheumatoid synovial fibroblasts. Also, because motifs of CENP-E show homologies to jun and fos oncogene products and are involved in virus assembly, CENP-E may be involved in the pathophysiology of RA. |
| distribution |
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"title": "Official Government Data Source",
"mediaType": "text/html",
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|
| identifier | https://healthdata.gov/api/views/mriz-uz2x |
| issued | 2025-07-13 |
| keyword |
[
"cenp-e-protein",
"gene-expression",
"nih",
"rheumatoid-arthritis",
"synovial-fibroblasts"
]
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| landingPage | https://healthdata.gov/d/mriz-uz2x |
| modified | 2025-09-06 |
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| publisher |
{
"name": "National Institutes of Health",
"@type": "org:Organization"
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| theme |
[
"NIH"
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|
| title | Kinesin-like protein CENP-E is upregulated in rheumatoid synovial fibroblasts |
