LAMP assay for detection of Asian fish tapeworm
Data are relative fluorescent units obtained from the FAM channel of a AmpliFire Isothermal Fluorometer (Agdia, Inc., Elkhart, USA) recorded every 10 sec during as isothermal (LAMP) amplification. The isothermal reaction is to detect the Asian fish tapeworm, Schyzocotyle acheilognathi [syn. Bothriocephalus]. Samples (column headers) are described in tabs and include 1) positive control dilution series, 2) DNA Extractions from AFT sensitivity samples, and 3) DNA Extracted from non-AFT specificity controls.
Complete Metadata
| accessLevel | public |
|---|---|
| bureauCode |
[
"010:12"
]
|
| contactPoint |
{
"fn": "Erik K Hofmeister",
"@type": "vcard:Contact",
"hasEmail": "mailto:ehofmeister@usgs.gov"
}
|
| description | Data are relative fluorescent units obtained from the FAM channel of a AmpliFire Isothermal Fluorometer (Agdia, Inc., Elkhart, USA) recorded every 10 sec during as isothermal (LAMP) amplification. The isothermal reaction is to detect the Asian fish tapeworm, Schyzocotyle acheilognathi [syn. Bothriocephalus]. Samples (column headers) are described in tabs and include 1) positive control dilution series, 2) DNA Extractions from AFT sensitivity samples, and 3) DNA Extracted from non-AFT specificity controls. |
| distribution |
[
{
"@type": "dcat:Distribution",
"title": "Digital Data",
"format": "XML",
"accessURL": "https://doi.org/10.5066/P96BOEN3",
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},
{
"@type": "dcat:Distribution",
"title": "Original Metadata",
"format": "XML",
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"downloadURL": "https://data.usgs.gov/datacatalog/metadata/USGS.5f862b1882cebef40f14c7a0.xml"
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|
| identifier | http://datainventory.doi.gov/id/dataset/USGS_5f862b1882cebef40f14c7a0 |
| keyword |
[
"Asian fish tapeworm",
"LAMP assay",
"USGS:5f862b1882cebef40f14c7a0",
"eDNA",
"environmental DNA",
"invasive species",
"isothermal amplification",
"loop-mediated isothermal amplification",
"molecular detection",
"parasites",
"rapid assay"
]
|
| modified | 2021-10-12T00:00:00Z |
| publisher |
{
"name": "U.S. Geological Survey",
"@type": "org:Organization"
}
|
| spatial | 0.0000, 0.0000, 0.0000, 0.0000 |
| theme |
[
"geospatial"
]
|
| title | LAMP assay for detection of Asian fish tapeworm |
