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Measurement Quality Metrics to Improve Absolute Microbial Cell Counting
This repository contains the raw data and analysis scripts supporting the associated publication which introduces a framework to help researchers select fit-for-purpose microbial cell counting methods and optimize protocols for quantification of microbial total cells and viable cells. Escherichia coli cells were enumerated using four methods (colony forming unit assay, impedance flow cytometry - Multisizer 4, impedance flow cytometry - BactoBox, and fluorescent flow cytometry - CytoFLEX LX) and repeated on multiple dates. The experimental design for a single date starts with a cell stock that is divided into 18 sample replicates (3 each for 6 different dilution factors), and each sample is assayed one or two times for a total of 30 observations. Raw data files are provided from the Multisizer 4 (*.#m4) and CytoFLEX LX (*.fcs 3.0). The colony forming unit assay and BactoBox readings are recorded for each date as are the derived results from the Multisizer 4 and CytoFLEX LX. Also provided are an example analysis script for the *.fcs files and the statistical analysis that was performed.
Complete Metadata
| @type | dcat:Dataset |
|---|---|
| accessLevel | public |
| accrualPeriodicity | irregular |
| bureauCode |
[
"006:55"
]
|
| contactPoint |
{
"fn": "Kirsten Parratt",
"hasEmail": "mailto:kirsten.parratt@nist.gov"
}
|
| description | This repository contains the raw data and analysis scripts supporting the associated publication which introduces a framework to help researchers select fit-for-purpose microbial cell counting methods and optimize protocols for quantification of microbial total cells and viable cells. Escherichia coli cells were enumerated using four methods (colony forming unit assay, impedance flow cytometry - Multisizer 4, impedance flow cytometry - BactoBox, and fluorescent flow cytometry - CytoFLEX LX) and repeated on multiple dates. The experimental design for a single date starts with a cell stock that is divided into 18 sample replicates (3 each for 6 different dilution factors), and each sample is assayed one or two times for a total of 30 observations. Raw data files are provided from the Multisizer 4 (*.#m4) and CytoFLEX LX (*.fcs 3.0). The colony forming unit assay and BactoBox readings are recorded for each date as are the derived results from the Multisizer 4 and CytoFLEX LX. Also provided are an example analysis script for the *.fcs files and the statistical analysis that was performed. |
| distribution |
[
{
"title": "AnalysisScripts",
"format": "Files are (*.R) and (*.RMD)",
"mediaType": "application/x-zip-compressed",
"description": "Analysis scripts for processing raw data and statistical analysis as described in the manuscript.",
"downloadURL": "https://data.nist.gov/od/ds/mds2-3410/AnalysisScripts.zip"
},
{
"title": "BioRep1 Data",
"format": "CytoFLEX (.fcs) files",
"mediaType": "application/x-zip-compressed",
"description": "Data from BioRep1 described in the manuscript.",
"downloadURL": "https://data.nist.gov/od/ds/mds2-3410/BioRep1.zip"
},
{
"title": "BioRep2 Data",
"format": "CytoFLEX (.fcs) and Multisizer (.#m4) files",
"mediaType": "application/x-zip-compressed",
"description": "Data from BioRep2 described in the manuscript",
"downloadURL": "https://data.nist.gov/od/ds/mds2-3410/BioRep2.zip"
},
{
"title": "BioRep3 Data",
"format": "CytoFLEX (.fcs) and Multisizer (.#m4) files",
"mediaType": "application/x-zip-compressed",
"description": "Data from BioRep3 described in the manuscript",
"downloadURL": "https://data.nist.gov/od/ds/mds2-3410/BioRep3.zip"
},
{
"title": "BeadRep Data",
"format": "Multisizer (.#m4) and CytoFLEX (.fcs) files",
"mediaType": "application/x-zip-compressed",
"description": "Data from the Bead experiment described in the manuscript",
"downloadURL": "https://data.nist.gov/od/ds/mds2-3410/BeadRep.zip"
},
{
"title": "CollatedReportingSpreadsheet",
"mediaType": "text/csv",
"downloadURL": "https://data.nist.gov/od/ds/mds2-3410/CollatedReportingSpreadsheet.csv"
},
{
"title": "README",
"mediaType": "text/plain",
"downloadURL": "https://data.nist.gov/od/ds/mds2-3410/README.txt"
}
]
|
| identifier | ark:/88434/mds2-3410 |
| issued | 2025-02-13 |
| keyword |
[
"Microbial; Measurement Quality Metric; Viability; Cell Counting"
]
|
| landingPage | https://data.nist.gov/od/id/mds2-3410 |
| language |
[
"en"
]
|
| license | https://www.nist.gov/open/license |
| modified | 2024-07-15 00:00:00 |
| programCode |
[
"006:045"
]
|
| publisher |
{
"name": "National Institute of Standards and Technology",
"@type": "org:Organization"
}
|
| theme |
[
"Bioscience:Microbial measurements"
]
|
| title | Measurement Quality Metrics to Improve Absolute Microbial Cell Counting |
