Measurements of SARS-CoV-2 and target concentrations in wastewater near Cincinnati, OH, from May to October 2020.
This dataset contains the raw droplet counts resulting from of ddPCR or RT-ddPCR of nucleic acid extracts from wastewater. The wastewater was collected from three different sewersheds in Southwest Ohio (Mill Creek WWTP, Taylor Creek WWTP, and a sub-sewershed, Lick Run). ddPCR counts (positive droplets and total droplets) are provided for the following targets: N1 and N2 (SARS-CoV-2 nucleocapsid genes), crAssphage, PMMoV, HF183 (all fecal indicators), and OC43 (an RNA spike-in from a cultured coronavirus). Other metadata (pH, flow, temperature, TSS, CBOD5) are provided where available.
This dataset is associated with the following publication:
Nagarkar, M., S. Keely, M. Jahne, E. Wheaton, C. Hart, B. Smith, J. Garland, E. Varughese, A. Braam, B. Wiechman, B. Morris, and N. Brinkman. SARS-CoV-2 Monitoring at three sewersheds of different scales and complexity demonstrates distinctive relationships between wastewater measurements and COVID-19 case data. SCIENCE OF THE TOTAL ENVIRONMENT. Elsevier BV, AMSTERDAM, NETHERLANDS, 816: 151534, (2022).
Complete Metadata
| accessLevel | public |
|---|---|
| bureauCode |
[
"020:00"
]
|
| contactPoint |
{
"fn": "Maitreyi Nagarkar",
"hasEmail": "mailto:nagarkar.maitreyi@epa.gov"
}
|
| description | This dataset contains the raw droplet counts resulting from of ddPCR or RT-ddPCR of nucleic acid extracts from wastewater. The wastewater was collected from three different sewersheds in Southwest Ohio (Mill Creek WWTP, Taylor Creek WWTP, and a sub-sewershed, Lick Run). ddPCR counts (positive droplets and total droplets) are provided for the following targets: N1 and N2 (SARS-CoV-2 nucleocapsid genes), crAssphage, PMMoV, HF183 (all fecal indicators), and OC43 (an RNA spike-in from a cultured coronavirus). Other metadata (pH, flow, temperature, TSS, CBOD5) are provided where available. This dataset is associated with the following publication: Nagarkar, M., S. Keely, M. Jahne, E. Wheaton, C. Hart, B. Smith, J. Garland, E. Varughese, A. Braam, B. Wiechman, B. Morris, and N. Brinkman. SARS-CoV-2 Monitoring at three sewersheds of different scales and complexity demonstrates distinctive relationships between wastewater measurements and COVID-19 case data. SCIENCE OF THE TOTAL ENVIRONMENT. Elsevier BV, AMSTERDAM, NETHERLANDS, 816: 151534, (2022). |
| distribution |
[
{
"title": "SupplementaryTable3_072121.xlsx",
"mediaType": "application/vnd.openxmlformats-officedocument.spreadsheetml.sheet",
"downloadURL": "https://pasteur.epa.gov/uploads/10.23719/1522921/SupplementaryTable3_072121.xlsx"
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]
|
| identifier | https://doi.org/10.23719/1522921 |
| keyword |
[
"COVID-19 Surveillance",
"Centralized Wastewater Treatment System",
"Coronavirus Disease 2019",
"CrAssphage",
"Ohio",
"PMMoV",
"ddPCR",
"hf183",
"sewershed"
]
|
| license | https://pasteur.epa.gov/license/sciencehub-license.html |
| modified | 2021-07-21 |
| programCode |
[
"020:000"
]
|
| publisher |
{
"name": "U.S. EPA Office of Research and Development (ORD)",
"subOrganizationOf": {
"name": "U.S. Environmental Protection Agency",
"subOrganizationOf": {
"name": "U.S. Government"
}
}
}
|
| references |
[
"https://doi.org/10.1016/j.scitotenv.2021.151534",
"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8590472"
]
|
| rights |
null
|
| title | Measurements of SARS-CoV-2 and target concentrations in wastewater near Cincinnati, OH, from May to October 2020. |
