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Metropolitan DWDS Sequence Data Set

Published by U.S. EPA Office of Research and Development (ORD) | U.S. Environmental Protection Agency | Metadata Last Checked: August 02, 2025 | Last Modified: 2014-10-06
PS_GenBank.fasta file contain the sequences of the bacterial 16S rRNA-encoding gene for each representative sequence. The sequence containing four hypervariable regions was amplified using the primer set Eub-8f and 787. PS_GenBank.xlsx file contains rows as sample and columns as entries representing the accession number (NCBI) deposited in GenBank for each representative sequence (i.e. unique sequences). PS_OTU.fasta file contain the sequences of the bacterial 16S rRNA-encoding gene for each Operational Taxonomic Unit (OTU). The sequence containing four hypervariable regions was amplified using the primer set Eub-8f and 787. This dataset is associated with the following publication: Revetta , R., V. Gomez-Alvarez, T. Gerke, J. Santodomingo , and N. Ashbolt. CHANGES IN BACTERIAL COMPOSITION OF BIOFILM IN A METROPOLITAN DRINKING WATER DISTRIBUTION SYSTEM. JOURNAL OF APPLIED MICROBIOLOGY. Blackwell Publishing, Malden, MA, USA, 121(1): 294-305, (2016).

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