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Molecular and morphological data to resolve species boundaries in the critically imperiled freshwater mussel species, Fusconaia mitchelli

Published by U.S. Geological Survey | Department of the Interior | Metadata Last Checked: January 27, 2026 | Last Modified: 2020-08-30T00:00:00Z
We generated multilocus DNA sequence data and traditional morphometric measurements to evaluate species boundaries in Fusconaia mitchelli. We sequenced three loci: the protein-coding mitochondrial DNA genes cytochrome oxidase subunit 1 and NADH dehydrogenase 1, and the nuclear ribosomal internal transcribed spacer 1. We also took three measurements, maximum length, maximum width, and maximum height, of mussel shells for morphometric analysis.

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