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Mosaic Standards Challenge

Published by National Institute of Standards and Technology | National Institute of Standards and Technology | Metadata Last Checked: August 02, 2025 | Last Modified: 2020-01-13 00:00:00

This repository contains all submissions made to the Mosaic Standards challenge from the period of the challenge opening through December 31st, 2019. The Mosaic Standards Challenge asked the microbiome research community to participate in determining the level of variation due to wet-lab protocols by sequencing a set of samples and providing the resulting files. Each participant ordered one or more kits, where each kit contained five fecal samples and two predetermined DNA mixtures. All samples were identical across all kits; in other words, the samples labled "#1" provided to each lab were identical to each other. Participants in the challenge sequenced any number of the provided samples and provided both the raw sequencing result files, and the details of their protocol. Protocol details were provided by answering a set of pre-specified questions in a metadata spreadsheet upon submission of each sample.

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microbiome; methodology; metagenomic sequencing;

Complete Metadata

@type	dcat:Dataset
accessLevel	public
accrualPeriodicity	irregular
bureauCode	[ "006:55" ]
contactPoint	{ "fn": "Samuel P. Forry", "hasEmail": "mailto:samuel.forry@nist.gov" }
description	This repository contains all submissions made to the Mosaic Standards challenge from the period of the challenge opening through December 31st, 2019. The Mosaic Standards Challenge asked the microbiome research community to participate in determining the level of variation due to wet-lab protocols by sequencing a set of samples and providing the resulting files. Each participant ordered one or more kits, where each kit contained five fecal samples and two predetermined DNA mixtures. All samples were identical across all kits; in other words, the samples labled "#1" provided to each lab were identical to each other. Participants in the challenge sequenced any number of the provided samples and provided both the raw sequencing result files, and the details of their protocol. Protocol details were provided by answering a set of pre-specified questions in a metadata spreadsheet upon submission of each sample.
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identifier	ark:/88434/mds2-2830
keyword	[ "microbiome; methodology; metagenomic sequencing;" ]
landingPage	https://data.nist.gov/od/id/mds2-2830
language	[ "en" ]
license	https://www.nist.gov/open/license
modified	2020-01-13 00:00:00
programCode	[ "006:045" ]
publisher	{ "name": "National Institute of Standards and Technology", "@type": "org:Organization" }
theme	[ "Bioscience:Microbial measurements" ]
title	Mosaic Standards Challenge