mRNA expression profile in DLD-1 and MOLT-4 cancer cell lines cultured under Microgravity
DLD-1 and MOLT-4 cell lines were cultured in a rotating cell culture system to simulate microgravity and mRNA expression profile in comparison to Static controls. Cells were grown in 10mL rotating vessels in a Rotary Cell Culture System (RCCS) and in 60mm Petri dishes (test control respectively). Two replicates of test (Microgravity) and control (static) each from DLD-1 and MOLT-4 were analyzed by microarray. Simulated microgravity affected the solid tumor cell line DLD-1 markedly which showed a higher percentage of dysregulated genes compared to the hematological tumor cell line MOLT-4. Microgravity affects the cell cycle of DLD-1 cells and disturbs expression of cell cycle regulatory gene networks. Multiple microRNA host genes were dysregulated and significantly mir-22 tumor suppressor microRNA is highly upregulated in DLD-1.
Complete Metadata
| @type | dcat:Dataset |
|---|---|
| accessLevel | public |
| accrualPeriodicity | irregular |
| bureauCode |
[
"026:00"
]
|
| contactPoint |
{
"fn": "GeneLab Outreach",
"@type": "vcard:Contact",
"hasEmail": "mailto:genelab-outreach@lists.nasa.gov"
}
|
| description | DLD-1 and MOLT-4 cell lines were cultured in a rotating cell culture system to simulate microgravity and mRNA expression profile in comparison to Static controls. Cells were grown in 10mL rotating vessels in a Rotary Cell Culture System (RCCS) and in 60mm Petri dishes (test control respectively). Two replicates of test (Microgravity) and control (static) each from DLD-1 and MOLT-4 were analyzed by microarray. Simulated microgravity affected the solid tumor cell line DLD-1 markedly which showed a higher percentage of dysregulated genes compared to the hematological tumor cell line MOLT-4. Microgravity affects the cell cycle of DLD-1 cells and disturbs expression of cell cycle regulatory gene networks. Multiple microRNA host genes were dysregulated and significantly mir-22 tumor suppressor microRNA is highly upregulated in DLD-1. |
| distribution |
[
{
"@type": "dcat:Distribution",
"title": "mRNA expression profile in DLD-1 and MOLT-4 cancer cell lines cultured under Microgravity",
"format": "HTML",
"mediaType": "text/html",
"description": "GeneLab Study Page",
"downloadURL": "https://genelab-data.ndc.nasa.gov/genelab/accession/GLDS-125"
}
]
|
| identifier | nasa_genelab_GLDS-125_um5r-gqim |
| issued | 2021-05-21 |
| keyword |
[
"cell-line",
"data-collection",
"data-transformation",
"genelab-microarray-data-processing-protocol",
"growth-protocol",
"labeling",
"microgravity-simulation",
"normalization-data-transformation",
"nucleic-acid-hybridization",
"rna-extraction"
]
|
| landingPage | https://data.nasa.gov/dataset/mrna-expression-profile-in-dld-1-and-molt-4-cancer-cell-lines-cultured-under-microgravity |
| license | http://www.usa.gov/publicdomain/label/1.0/ |
| modified | 2025-04-23 |
| programCode |
[
"026:005"
]
|
| publisher |
{
"name": "National Aeronautics and Space Administration",
"@type": "org:Organization"
}
|
| theme |
[
"Earth Science"
]
|
| title | mRNA expression profile in DLD-1 and MOLT-4 cancer cell lines cultured under Microgravity |
