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Mucket eDNA detection in Wallen’s Bend, Clinch River, Tennessee, September 2019

Published by U.S. Geological Survey | Department of the Interior | Metadata Last Checked: January 27, 2026 | Last Modified: 2020-11-19T00:00:00Z
The data describe the technical performance of a designed qPCR assay for the detection of mucket (Actinonaias ligamentina) eDNA from field collected water samples. Parameters described include the sequences of the primers and probes used; the limit of detection and limit of quantification for the assay; a list of freshwater mussels species that were used to test specificity of the assay; and in situ field samples that were tested to the verify assay.

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