Multi-omic investigations of mouse liver subjected to simulated spaceflight freezing and storage protocols
This study compares standard laboratory protocols for tissue freezing and storage with a simulation of the delayed processing of liver specimens and long-term storage protocols used during the Rodent Research-1 (RR-1) payload. Liver samples from twelve-week old female C57/BL6 mice (snap frozen vs. 25 min. slow-frozen and stored for 3 days vs. 1 year) received from the RR-1 project were processed for extraction of RNA DNA and protein. RNA-seq whole genome bisulfite sequencing and mass spectrometry proteomics were performed. These multi-omics datasets will help distinguish the effects of freezing and storage time that could confound the interpretation of the Rodent Research-1 datasets.
Complete Metadata
| @type | dcat:Dataset |
|---|---|
| accessLevel | public |
| accrualPeriodicity | irregular |
| bureauCode |
[
"026:00"
]
|
| contactPoint |
{
"fn": "GeneLab Outreach",
"@type": "vcard:Contact",
"hasEmail": "mailto:genelab-outreach@lists.nasa.gov"
}
|
| description | This study compares standard laboratory protocols for tissue freezing and storage with a simulation of the delayed processing of liver specimens and long-term storage protocols used during the Rodent Research-1 (RR-1) payload. Liver samples from twelve-week old female C57/BL6 mice (snap frozen vs. 25 min. slow-frozen and stored for 3 days vs. 1 year) received from the RR-1 project were processed for extraction of RNA DNA and protein. RNA-seq whole genome bisulfite sequencing and mass spectrometry proteomics were performed. These multi-omics datasets will help distinguish the effects of freezing and storage time that could confound the interpretation of the Rodent Research-1 datasets. |
| distribution |
[
{
"@type": "dcat:Distribution",
"title": "Multi-omic investigations of mouse liver subjected to simulated spaceflight freezing and storage protocols",
"format": "HTML",
"mediaType": "text/html",
"description": "GeneLab Study Page",
"downloadURL": "https://genelab-data.ndc.nasa.gov/genelab/accession/GLDS-49"
}
]
|
| identifier | nasa_genelab_GLDS-49_56j4-37dc |
| issued | 2018-06-26 |
| keyword |
[
"animal-and-tissue-sampling",
"extraction",
"freezing-profile",
"labeling",
"library-construction",
"mass-spectrometry",
"nucleic-acid-extraction",
"nucleic-acid-sequencing",
"protein-extraction",
"proteomic-labeling",
"sample-collection",
"tissue-storage-time"
]
|
| landingPage | https://data.nasa.gov/dataset/multi-omic-investigations-of-mouse-liver-subjected-to-simulated-spaceflight-freezing-and-s |
| modified | 2025-04-23 |
| programCode |
[
"026:005"
]
|
| publisher |
{
"name": "National Aeronautics and Space Administration",
"@type": "org:Organization"
}
|
| theme |
[
"Earth Science"
]
|
| title | Multi-omic investigations of mouse liver subjected to simulated spaceflight freezing and storage protocols |
