Nitrogen and nitrogen isotope data for wheat and barley exposed to CeO2 nanoparticles
The effects of cerium oxide nanoparticles (CeO2-NPs) on 15N/14N ratio (δ15N) in wheat and barley were investigated. Seedlings were exposed to 0 and 500 mg CeO2-NPs/L (Ce-0 and Ce-500, respectively) in hydroponic suspension supplied with NH4NO3, NH4+, or NO3-. N uptake and δ15N discrimination (i.e. differences in δ15N of plant and δ15N of N source) were measured. Results showed that N content and 15N abundance decreased in wheat but increased in barley. Ce-500 only induced whole-plant δ15N discrimination (-1.48‰, P ≤ 0.10) with a simultaneous decrease (P ≤ 0.05) in whole-plant δ15N (-3.24‰) compared to Ce-0 (-2.74‰) in wheat in NH4+. Ce-500 decreased (P ≤ 0.01) root δ15N of wheat in NH4NO3 and NH4+ (3.23 and -2.25‰, respectively) compared to Ce-0 (4.96 and -1.27‰, respectively), but increased (P ≤ 0.05) root δ15N of wheat in NO3- (3.27‰) compared to Ce-0 (2.60‰). Synchrotron micro-XRF revealed the presence of CeO2-NPs in shoots of wheat and barley regardless of N source. Although the longer-term consequences of CeO2-NP exposure on N uptake and metabolism are unknown, the results clearly show the potential for ENMs to interfere with plant metabolism of critical plant nutrients such as N even when toxicity is not observed.
This dataset is associated with the following publication:
Rico, C.M., M. Johnson, M.A. Marcus, and C.P. Andersen. Shifts in N and δ15N in wheat and barley exposed to cerium oxide nanoparticles. NanoImpact. Elsevier B.V., Amsterdam, NETHERLANDS, 11: 156-163, (2018).
Complete Metadata
| accessLevel | public |
|---|---|
| bureauCode |
[
"020:00"
]
|
| contactPoint |
{
"fn": "Christian Andersen",
"hasEmail": "mailto:andersen.christian@epa.gov"
}
|
| description | The effects of cerium oxide nanoparticles (CeO2-NPs) on 15N/14N ratio (δ15N) in wheat and barley were investigated. Seedlings were exposed to 0 and 500 mg CeO2-NPs/L (Ce-0 and Ce-500, respectively) in hydroponic suspension supplied with NH4NO3, NH4+, or NO3-. N uptake and δ15N discrimination (i.e. differences in δ15N of plant and δ15N of N source) were measured. Results showed that N content and 15N abundance decreased in wheat but increased in barley. Ce-500 only induced whole-plant δ15N discrimination (-1.48‰, P ≤ 0.10) with a simultaneous decrease (P ≤ 0.05) in whole-plant δ15N (-3.24‰) compared to Ce-0 (-2.74‰) in wheat in NH4+. Ce-500 decreased (P ≤ 0.01) root δ15N of wheat in NH4NO3 and NH4+ (3.23 and -2.25‰, respectively) compared to Ce-0 (4.96 and -1.27‰, respectively), but increased (P ≤ 0.05) root δ15N of wheat in NO3- (3.27‰) compared to Ce-0 (2.60‰). Synchrotron micro-XRF revealed the presence of CeO2-NPs in shoots of wheat and barley regardless of N source. Although the longer-term consequences of CeO2-NP exposure on N uptake and metabolism are unknown, the results clearly show the potential for ENMs to interfere with plant metabolism of critical plant nutrients such as N even when toxicity is not observed. This dataset is associated with the following publication: Rico, C.M., M. Johnson, M.A. Marcus, and C.P. Andersen. Shifts in N and δ15N in wheat and barley exposed to cerium oxide nanoparticles. NanoImpact. Elsevier B.V., Amsterdam, NETHERLANDS, 11: 156-163, (2018). |
| distribution |
[
{
"title": "hydroponics NH barley Isotope - data repository for Chris.xlsx",
"mediaType": "application/vnd.openxmlformats-officedocument.spreadsheetml.sheet",
"downloadURL": "https://pasteur.epa.gov/uploads/10.23719/1411219/hydroponics%20NH%20barley%20Isotope%20-%20data%20repository%20for%20Chris.xlsx"
},
{
"title": "hydroponics NH wheat Isotope - data repository for Chris.xlsx",
"mediaType": "application/vnd.openxmlformats-officedocument.spreadsheetml.sheet",
"downloadURL": "https://pasteur.epa.gov/uploads/10.23719/1411219/hydroponics%20NH%20wheat%20Isotope%20-%20data%20repository%20for%20Chris.xlsx"
},
{
"title": "hydroponics NHNO barley Isotope - data repository for Chris.xlsx",
"mediaType": "application/vnd.openxmlformats-officedocument.spreadsheetml.sheet",
"downloadURL": "https://pasteur.epa.gov/uploads/10.23719/1411219/hydroponics%20NHNO%20barley%20Isotope%20-%20data%20repository%20for%20Chris.xlsx"
},
{
"title": "hydroponics NHNO wheat Isotope - data repository for Chris.xlsx",
"mediaType": "application/vnd.openxmlformats-officedocument.spreadsheetml.sheet",
"downloadURL": "https://pasteur.epa.gov/uploads/10.23719/1411219/hydroponics%20NHNO%20wheat%20Isotope%20-%20data%20repository%20for%20Chris.xlsx"
},
{
"title": "hydroponics NO barley Isotope - data repository for Chris.xlsx",
"mediaType": "application/vnd.openxmlformats-officedocument.spreadsheetml.sheet",
"downloadURL": "https://pasteur.epa.gov/uploads/10.23719/1411219/hydroponics%20NO%20barley%20Isotope%20-%20data%20repository%20for%20Chris.xlsx"
},
{
"title": "hydroponics NO wheat Isotope - data repository for Chris.xlsx",
"mediaType": "application/vnd.openxmlformats-officedocument.spreadsheetml.sheet",
"downloadURL": "https://pasteur.epa.gov/uploads/10.23719/1411219/hydroponics%20NO%20wheat%20Isotope%20-%20data%20repository%20for%20Chris.xlsx"
}
]
|
| identifier | https://doi.org/10.23719/1411219 |
| keyword |
[
"Synchrotron micro XRF",
"ammonium nitrate",
"engineered nanomaterials (ENMs)",
"intergenerational effects",
"isotope",
"isotopic discrimination",
"nitrogen"
]
|
| license | https://pasteur.epa.gov/license/sciencehub-license.html |
| modified | 2018-03-15 |
| programCode |
[
"020:095"
]
|
| publisher |
{
"name": "U.S. EPA Office of Research and Development (ORD)",
"subOrganizationOf": {
"name": "U.S. Environmental Protection Agency",
"subOrganizationOf": {
"name": "U.S. Government"
}
}
}
|
| references |
[
"https://doi.org/10.1016/j.impact.2018.08.003"
]
|
| rights |
null
|
| title | Nitrogen and nitrogen isotope data for wheat and barley exposed to CeO2 nanoparticles |
