Oyster Larval Survival Counts from Probiotic OY15 Experiments
Environmentally-friendly methods for controlling microbial pathogenesis in aquaculture with probiotic bacteria are becoming increasingly preferred over use of chemical means, such as disinfectants or antibiotics. Recent research at the Milford Laboratory has shown that naturally-occurring bacteria isolated from the digestive glands of adult oysters, Crassostrea virginica, may be used as potential probiotic candidates in oyster larviculture. Probiotic candidate selection was based upon the Kirby Bauer Disc Diffusion Method, in which zones of inhibition suggested competitive exclusion of a known shellfish larvae pathogen (B183). In challenge studies, survival of 2-day old oyster larvae supplemented with probiotic candidate OY15 (Vibrio sp.) was similar (p<0.3883) to that of control larvae (no added bacteria), indicating no harmful effects. Further, addition of OY15 to oyster larvae challenged with B183 significantly improved survival (p<0.0141) compared to the pathogen alone. An in vitro study to determine the effects of OY15 or the pathogen B183 upon oyster hemocytes and their immune functions demonstrated immuno-stimulation of oyster hemocytes by OY15 and immune-suppression by B183, suggesting an immuno-modulation mechanism for OY15.
Complete Metadata
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| description | Environmentally-friendly methods for controlling microbial pathogenesis in aquaculture with probiotic bacteria are becoming increasingly preferred over use of chemical means, such as disinfectants or antibiotics. Recent research at the Milford Laboratory has shown that naturally-occurring bacteria isolated from the digestive glands of adult oysters, Crassostrea virginica, may be used as potential probiotic candidates in oyster larviculture. Probiotic candidate selection was based upon the Kirby Bauer Disc Diffusion Method, in which zones of inhibition suggested competitive exclusion of a known shellfish larvae pathogen (B183). In challenge studies, survival of 2-day old oyster larvae supplemented with probiotic candidate OY15 (Vibrio sp.) was similar (p<0.3883) to that of control larvae (no added bacteria), indicating no harmful effects. Further, addition of OY15 to oyster larvae challenged with B183 significantly improved survival (p<0.0141) compared to the pathogen alone. An in vitro study to determine the effects of OY15 or the pathogen B183 upon oyster hemocytes and their immune functions demonstrated immuno-stimulation of oyster hemocytes by OY15 and immune-suppression by B183, suggesting an immuno-modulation mechanism for OY15. |
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| identifier | gov.noaa.nmfs.inport:26979 |
| issued | 2011-01-01T00:00:00.000+00:00 |
| keyword |
[
"Crassostrea virginica",
"Vibrio",
"disease prevention",
"larvae survival",
"oyster larviculture",
"probiotic bacteria",
"shellfish larvae",
"spring",
"Northeastern United States",
"Shellfish hatcheries",
"DOC/NOAA/NMFS/NEFSC > Northeast Fisheries Science Center, National Marine Fisheries Service, NOAA, U.S. Department of Commerce",
"Aquaculture Sustainability Branch (ASB)"
]
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[]
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| license | https://creativecommons.org/publicdomain/zero/1.0/ |
| modified | 2011-01-01T00:00:00.000+00:00 |
| publisher |
{
"name": "Northeast Fisheries Science Center",
"@type": "org:Organization"
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[
"https://www.fisheries.noaa.gov/inportserve/waf/noaa/nmfs/nefsc/dmp/pdf/26979.pdf"
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| rights | otherRestrictions, unclassified |
| spatial | -73.043572,41.212161,-73.053572,41.222161 |
| temporal | 2006-01-01T00:00:00+00:00/2011-01-01T00:00:00+00:00 |
| title | Oyster Larval Survival Counts from Probiotic OY15 Experiments |
