Patent AT-E401411-T1: [Translated] ßFROG-PRINCEß - A TRANSPOSON VECTOR FOR GENE TRANSFER IN VERTEBRATES

The present invention relates to a transposon-based DNA integration system comprising (a) a transposon which is devoid of a polynucleotide encoding a functional transposase and which comprises a polynucleotide of interest, wherein the transposon comprises inverted repeats having a degree of identity with the repeats within SEQ ID NO: 2 and its inverted repeat, respectively, of at least 90%; and (b) a transposase having at its N-terminus a DNA binding domain comprising the sequences of SEQ ID NO: 3 and 4; or (c) a polynucleotide encoding the transposase of (b). The present invention further relates to a method of transferring a polynucleotide of interest into cells of a vertebrate comprising the step of introducing the transposon-based DNA integration system of the invention into said cells. In addition, the invention relates to a method of effecting RNAi comprising (a) stably introducing a transposon comprising an expression cassette expressing a short interfering RNA and a selectable marker gene as part of the transposon-based DNA integration system of the invention into a cell; (b) selecting for cells expressing the selectable marker; and (c) assessing whether the transcription/translation of the desired gene is effected by RNAi. A further embodiment of the invention is a method of gene trapping genes comprising the steps of (a) introducing the transposon-based DNA integration system of the invention into a cell; and (b) assessing for the expression of a selectable marker wherein expression of a selectable marker is indicative of integration of the transposon into a transcibed gene of the cell.

                                            [
  "009:25"
]
                                        

                                            {
  "fn": "NIH/NCBI Data Team",
  "@type": "vcard:Contact",
  "hasEmail": "mailto:info@ncbi.nlm.nih.gov"
}
                                        

                                            [
  {
    "@type": "dcat:Distribution",
    "title": "Official Data Source",
    "mediaType": "text/html",
    "description": "Access the complete Patent AT-E401411-T1: [Translated] ßFROG-PRINCEß - A TRANSPOSON VECTOR FOR GENE TRANSFER IN VERTEBRATES on the official website.",
    "downloadURL": "https://pubchem.ncbi.nlm.nih.gov/patent/AT-E401411-T1"
  }
]
                                        

                                            [
  "chemistry",
  "innovation",
  "patent",
  "pubchem",
  "research"
]
                                        

                                            [
  "009:066"
]
                                        

                                            {
  "name": "National Center for Biotechnology Information (NCBI)",
  "@type": "org:Organization"
}
                                        

                                            [
  "NIH"
]