Polyacrylamide gel electrophoresis results with thiaminase activity stain of recombinant putative thiaminases expressed in E. coli
Data describe results of a set of laboratory experiments using polyacrylamide gel electrophoresis with a thiaminase activity stain to test the solubility and thiaminase activity of recombinant putative thiaminases expressed in E. coli. Parameters described include the presence and relative mobility of thiaminases, and the presence and relative mobility of recombinant proteins. Experiments were conducted at the USGS Columbia Environmental Research Center, Columbia, MO in 2012 and 2013.
Complete Metadata
| accessLevel | public |
|---|---|
| bureauCode |
[
"010:12"
]
|
| contactPoint |
{
"fn": "Cathy A Richter",
"@type": "vcard:Contact",
"hasEmail": "mailto:crichter@usgs.gov"
}
|
| description | Data describe results of a set of laboratory experiments using polyacrylamide gel electrophoresis with a thiaminase activity stain to test the solubility and thiaminase activity of recombinant putative thiaminases expressed in E. coli. Parameters described include the presence and relative mobility of thiaminases, and the presence and relative mobility of recombinant proteins. Experiments were conducted at the USGS Columbia Environmental Research Center, Columbia, MO in 2012 and 2013. |
| distribution |
[
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"accessURL": "https://doi.org/10.5066/P9DGI5F5",
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| identifier | http://datainventory.doi.gov/id/dataset/USGS_62ebfb24d34eacf539724d5e |
| keyword |
[
"USGS:62ebfb24d34eacf539724d5e",
"alewife",
"biota",
"fish",
"polyacrylamide gel electrophoresis",
"thiaminase",
"thiamine deficiency complex (TDC)",
"zebrafish"
]
|
| modified | 2023-01-17T00:00:00Z |
| publisher |
{
"name": "U.S. Geological Survey",
"@type": "org:Organization"
}
|
| spatial | -92.284300, 38.910784, -92.274450, 38.912612 |
| theme |
[
"Geospatial"
]
|
| title | Polyacrylamide gel electrophoresis results with thiaminase activity stain of recombinant putative thiaminases expressed in E. coli |
