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Preliminary Assessment of Pacific Lamprey Environmental DNA Samples from Western Washington Rivers.
qPCR detection results for Pacific Lamprey eDNA surveys from Western Washington Rivers and Streams. Water samples were collected via filtration and shipped to the laboratory for analysis. DNA was extracted from filters using commercially available extraction kits. Lab staff used two existing Pacific Lamprey qPCR eDNA surveys to screen DNA extracts for the presence of Pacific Lamprey DNA. Eight molecular replicates were run for each samples. Results presented in this table show the number of positive detections out of 8 for each sample and the corresponding mean Cq values for each sample with a detection.
Resources
3 resources available
-
Pacific Lamprey eDNA Detection Summary Data 7-17-24_20250218.html
HTML -
Pacific Lamprey mdjson-20250218-150263.json
JSON -
Pacific Lamprey Summary Data_7-17-24.csv
CSV
Complete Metadata
| accessLevel | public |
|---|---|
| bureauCode |
[
"010:18"
]
|
| contactPoint |
{
"fn": "Todd Sutherland",
"@type": "vcard:Contact",
"hasEmail": "mailto:todd_sutherland@fws.gov"
}
|
| description | qPCR detection results for Pacific Lamprey eDNA surveys from Western Washington Rivers and Streams. Water samples were collected via filtration and shipped to the laboratory for analysis. DNA was extracted from filters using commercially available extraction kits. Lab staff used two existing Pacific Lamprey qPCR eDNA surveys to screen DNA extracts for the presence of Pacific Lamprey DNA. Eight molecular replicates were run for each samples. Results presented in this table show the number of positive detections out of 8 for each sample and the corresponding mean Cq values for each sample with a detection. |
| distribution |
[
{
"@type": "dcat:Distribution",
"title": "Pacific Lamprey eDNA Detection Summary Data 7-17-24_20250218.html",
"format": "html",
"mediaType": "text/html",
"description": "Pacific Lamprey eDNA Detection Dataset Metadata (HTML File)",
"downloadURL": "https://iris.fws.gov/APPS/ServCat/DownloadFile/267975?Reference=167905"
},
{
"@type": "dcat:Distribution",
"title": "Pacific Lamprey mdjson-20250218-150263.json",
"format": "json",
"mediaType": "application/json",
"description": "Pacific Lamprey mdEditor JSON File",
"downloadURL": "https://iris.fws.gov/APPS/ServCat/DownloadFile/267974?Reference=167905"
},
{
"@type": "dcat:Distribution",
"title": "Pacific Lamprey Summary Data_7-17-24.csv",
"format": "csv",
"mediaType": "text/csv",
"description": "Tabular dataset of qPCR results collected at 2 Pacific Lamprey eDNA markers used in this study. For each sample analyzed, the general sample location and the number of filters collected is given. Columns on the data sheet show the number of positive molecular replicates (out of 8) and the mean Cq value for each assay.",
"downloadURL": "https://iris.fws.gov/APPS/ServCat/DownloadFile/252815?Reference=167905"
}
]
|
| identifier | http://datainventory.doi.gov/id/dataset/FWS_ServCat_167905 |
| issued | 2024-07-31T00:00:00Z |
| keyword |
[
"Entosphenus tridentatus",
"General: Biology | Species | Fishes",
"General: Management | Natural Resources Management | Fisheries Management",
"Pacific Lamprey",
"eDNA",
"filtration",
"qPCR",
"species monitoring",
"three-toothed lamprey"
]
|
| landingPage | https://iris.fws.gov/APPS/ServCat/Reference/Profile/167905 |
| modified | 2024-07-31T00:00:00Z |
| programCode |
[
"010:028",
"010:094"
]
|
| publisher |
{
"name": "U.S. Fish and Wildlife Service",
"@type": "org:Organization"
}
|
| spatial | -124.794212,46.8005638,-122.382713,48.4151077 |
| temporal | 2023-01-01/2024-07-31 |
| theme |
[
"Tabular Dataset"
]
|
| title | Preliminary Assessment of Pacific Lamprey Environmental DNA Samples from Western Washington Rivers. |
