Quantitative polymerase chain reaction (qPCR): An alternative, rapid water quality monitoring tool at a National Park on Lake Michigan.
Data were collected to evaluate the efficacy of quantitative polymerase chain reaction (qPCR) as a rapid, alternative method for monitoring recreational water at select beaches and rivers of Sleeping Bear Dunes National Lakeshore (SLBE), in Empire, Michigan. Water samples were collected between August 4 and September 18, 2014 (N=297) from four locations (Esch Rd, Otter Creek, Platte Bay, and Platte River). The samples were analyzed for indicator bacteria, E. coli and enterococci, by both culture-based (membrane filtration, MF; traditional method) and non-culture based (qPCR; rapid method). Recreational water quality standards (RWQS) and beach action values (BAV) were used as indices to compare water quality standard exceedances for MF and qPCR methods; for enterococci: 70 colony-forming units (MF) and 1,000 calibrator cell equivalents and for E. coli: 235 colony-forming units (MF); currently, there are no established RWQS based for qPCR (i.e., CCE) results.
The CultureBasedData data include E. coli and enterococci culture-based bacterial counts. Data from the qpcr_v3 file includes results from quantitative polymerase chain reaction (qPCR) method for estimation of E. coli and enterococci counts by DNA-based methods. The Lat_Long file includes information regarding sampling sites at each of the four locations and their corresponding latitude and longitudes.
Complete Metadata
| accessLevel | public |
|---|---|
| bureauCode |
[
"010:12"
]
|
| contactPoint |
{
"fn": "Muruleedhara Byappanahalli",
"@type": "vcard:Contact",
"hasEmail": "mailto:byappan@usgs.gov"
}
|
| description | Data were collected to evaluate the efficacy of quantitative polymerase chain reaction (qPCR) as a rapid, alternative method for monitoring recreational water at select beaches and rivers of Sleeping Bear Dunes National Lakeshore (SLBE), in Empire, Michigan. Water samples were collected between August 4 and September 18, 2014 (N=297) from four locations (Esch Rd, Otter Creek, Platte Bay, and Platte River). The samples were analyzed for indicator bacteria, E. coli and enterococci, by both culture-based (membrane filtration, MF; traditional method) and non-culture based (qPCR; rapid method). Recreational water quality standards (RWQS) and beach action values (BAV) were used as indices to compare water quality standard exceedances for MF and qPCR methods; for enterococci: 70 colony-forming units (MF) and 1,000 calibrator cell equivalents and for E. coli: 235 colony-forming units (MF); currently, there are no established RWQS based for qPCR (i.e., CCE) results. The CultureBasedData data include E. coli and enterococci culture-based bacterial counts. Data from the qpcr_v3 file includes results from quantitative polymerase chain reaction (qPCR) method for estimation of E. coli and enterococci counts by DNA-based methods. The Lat_Long file includes information regarding sampling sites at each of the four locations and their corresponding latitude and longitudes. |
| distribution |
[
{
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"title": "Digital Data",
"format": "XML",
"accessURL": "https://doi.org/10.5066/F77P8XND",
"mediaType": "application/http",
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"title": "Original Metadata",
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"downloadURL": "https://data.usgs.gov/datacatalog/metadata/USGS.5a4cf831e4b0d05ee8c4cae0.xml"
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|
| identifier | http://datainventory.doi.gov/id/dataset/USGS_5a4cf831e4b0d05ee8c4cae0 |
| keyword |
[
"Lake Michigan",
"Sleeping Bear Dunes National Lakeshore",
"USGS:5a4cf831e4b0d05ee8c4cae0",
"bacteria",
"biota",
"freshwater ecosystems",
"polymerase chain reaction",
"water quality",
"water sampling"
]
|
| modified | 2025-01-24T00:00:00Z |
| publisher |
{
"name": "U.S. Geological Survey",
"@type": "org:Organization"
}
|
| spatial | -86.2077, 44.6857, -86.0374, 44.8086 |
| theme |
[
"Geospatial"
]
|
| title | Quantitative polymerase chain reaction (qPCR): An alternative, rapid water quality monitoring tool at a National Park on Lake Michigan. |
