Return to search results
Rare Earth Desorption Test with Citrate and Bicarbonate
LBT (lanthanide binding tag) cells were grown overnight in LB media with 0.05% Amp. 1:100 subculture taken from overnights, grown for 2 hours. LBT was induced with 0.002% arabinose added for 3 hours. REE adsorption was done by combining 350 ul (0.25% 1M MES, 12.5 uM Tb, and 12.5 uM La or Cu in sterile DI water) and 350 ul (LBT cells with OD = 1 in 10 mM MES), reacted for approx. 30 min.
Following adsorption, citrate and bicarbonate solutions were used in desorption to recover rare earth from cell surface, and to further separate REE from non-REEs. The samples were then centrifuged and a fraction of the supernatant was collected for ICP-MS analysis.
Complete Metadata
| @type | dcat:Dataset |
|---|---|
| accessLevel | public |
| bureauCode |
[
"019:20"
]
|
| contactPoint |
{
"fn": "Yongqin Jiao",
"@type": "vcard:Contact",
"hasEmail": "mailto:jiao1@llnl.gov"
}
|
| dataQuality |
true
|
| description | LBT (lanthanide binding tag) cells were grown overnight in LB media with 0.05% Amp. 1:100 subculture taken from overnights, grown for 2 hours. LBT was induced with 0.002% arabinose added for 3 hours. REE adsorption was done by combining 350 ul (0.25% 1M MES, 12.5 uM Tb, and 12.5 uM La or Cu in sterile DI water) and 350 ul (LBT cells with OD = 1 in 10 mM MES), reacted for approx. 30 min. Following adsorption, citrate and bicarbonate solutions were used in desorption to recover rare earth from cell surface, and to further separate REE from non-REEs. The samples were then centrifuged and a fraction of the supernatant was collected for ICP-MS analysis. |
| distribution |
[
{
"@type": "dcat:Distribution",
"title": "Desorption Data.xlsx",
"format": "xlsx",
"accessURL": "https://gdr.openei.org/files/965/GTO_data_deposit_092017_Desorption.xlsx",
"mediaType": "application/vnd.openxmlformats-officedocument.spreadsheetml.sheet",
"description": "Excel file detailing results of the bicarbonate and citrate desorption experiments. Plots of results included."
}
]
|
| DOI | 10.15121/1452718 |
| identifier | https://data.openei.org/submissions/7133 |
| issued | 2017-06-01T06:00:00Z |
| keyword |
[
"REE",
"adsorption",
"bicarbonate",
"brine",
"centrifuge",
"citrate",
"desorption",
"energy",
"geothermal",
"lanthanide binding tag",
"lbt",
"rare earth",
"resource recovery"
]
|
| landingPage | https://gdr.openei.org/submissions/965 |
| license | https://creativecommons.org/licenses/by/4.0/ |
| modified | 2018-06-14T16:28:49Z |
| programCode |
[
"019:006"
]
|
| projectLead | Holly Thomas |
| projectNumber | LLNL FY17 AOP 2.5.1.12 |
| projectTitle | Extraction of Rare Earth Metals from Geothermal Fluids using Bioengineered Microbes |
| publisher |
{
"name": "Lawrence Livermore National Laboratory",
"@type": "org:Organization"
}
|
| spatial |
"{"type":"Polygon","coordinates":[[[-122.2433640625,35.15607354867],[-118.3872640625,35.15607354867],[-118.3872640625,39.295415290912],[-122.2433640625,39.295415290912],[-122.2433640625,35.15607354867]]]}"
|
| title | Rare Earth Desorption Test with Citrate and Bicarbonate |
