Raw absorbance values from serially diluted uninfected whole blood in different lysis buffers for Hawaiian Island forest bird avian malaria detection, 2005-2006
It is unclear whether DNA lysis buffers used for preservation of whole blood samples from Hawaiian forest birds cause denaturation and loss of antigen binding capability of antibody molecules. If their antigen binding capability is not affected, then the samples can be used in serological assays to provide an independent assessment of the accuracy of PCR (polymerase chain reaction) diagnostic tests for avian malaria.
This data set provides raw absorbance measurements that were collected with a BioRad Model 3550 ELISA plate reader at a wavelength setting of 405 nm for different dilutions of blood from an uninfected Hawaii Amakihi. The blood was preserved in different lysis buffers or placed in phosphate buffered saline as an untreated control.
Complete Metadata
| accessLevel | public |
|---|---|
| bureauCode |
[
"010:12"
]
|
| contactPoint |
{
"fn": "Carter T Atkinson",
"@type": "vcard:Contact",
"hasEmail": "mailto:catkinson@usgs.gov"
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|
| description | It is unclear whether DNA lysis buffers used for preservation of whole blood samples from Hawaiian forest birds cause denaturation and loss of antigen binding capability of antibody molecules. If their antigen binding capability is not affected, then the samples can be used in serological assays to provide an independent assessment of the accuracy of PCR (polymerase chain reaction) diagnostic tests for avian malaria. This data set provides raw absorbance measurements that were collected with a BioRad Model 3550 ELISA plate reader at a wavelength setting of 405 nm for different dilutions of blood from an uninfected Hawaii Amakihi. The blood was preserved in different lysis buffers or placed in phosphate buffered saline as an untreated control. |
| distribution |
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|
| identifier | http://datainventory.doi.gov/id/dataset/USGS_5e98e24582ce172707f6f985 |
| keyword |
[
"Chlorodrepanis virens",
"ELISA",
"Hakalau Forest National Wildlife Refuge",
"Hawaii",
"Hawaii Amakihi",
"Hawaiian Islands",
"Lysis Buffer",
"PCR",
"Plasmodium relictum",
"USGS:5e98e24582ce172707f6f985",
"Western blot",
"avian malaria",
"biota",
"enzyme linked immunosorbant assay",
"immunoblot",
"polymerase chain reaction"
]
|
| modified | 2020-08-27T00:00:00Z |
| publisher |
{
"name": "U.S. Geological Survey",
"@type": "org:Organization"
}
|
| spatial | -156.2640, 18.8023, -154.6820, 20.3395 |
| theme |
[
"Geospatial"
]
|
| title | Raw absorbance values from serially diluted uninfected whole blood in different lysis buffers for Hawaiian Island forest bird avian malaria detection, 2005-2006 |
