Reporting the limits of detection (LOD) and quantification (LOQ) for environmental DNA assays: Data
This data set was collected to provide examples and aid in developing a standardized way of determining LOD and LOQ for eDNA assays and has 3 data files. GEDWG_LOD_DATA3.csv is raw qPCR data from multiple labs running multiple standards of known concentration for eDNA assays they regularly use. Comparison-Data.csv is the merged data output from running a generic LOD/LOQ calculator script multiple times with different LOD model settings. The generic LOD/LOQ calculator script is available at: https://github.com/cmerkes/qPCR_LOD_Calc, and details about the multiple settings used are commented in the analysis script available at: https://github.com/cmerkes/LOD_Analysis
Complete Metadata
| accessLevel | public |
|---|---|
| bureauCode |
[
"010:12"
]
|
| contactPoint |
{
"fn": "Christopher M Merkes",
"@type": "vcard:Contact",
"hasEmail": "mailto:cmerkes@usgs.gov"
}
|
| description | This data set was collected to provide examples and aid in developing a standardized way of determining LOD and LOQ for eDNA assays and has 3 data files. GEDWG_LOD_DATA3.csv is raw qPCR data from multiple labs running multiple standards of known concentration for eDNA assays they regularly use. Comparison-Data.csv is the merged data output from running a generic LOD/LOQ calculator script multiple times with different LOD model settings. The generic LOD/LOQ calculator script is available at: https://github.com/cmerkes/qPCR_LOD_Calc, and details about the multiple settings used are commented in the analysis script available at: https://github.com/cmerkes/LOD_Analysis |
| distribution |
[
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|
| identifier | http://datainventory.doi.gov/id/dataset/USGS_5d36fbfce4b01d82ce8a6cbf |
| keyword |
[
"Columbia Environmental Research Center, 4200 E New Haven Road, Columbia, Missouri, 65201",
"Department of Biochemistry and Microbiology, University of Victoria, 3800 Finnerty Road, Victoria, British Columbia, V8P 5C2, Canada",
"Engineers Research and Development Center, 3909 Halls Ferry Road, Vicksburg, Mississippi, 39180",
"Environmental DNA",
"National Wildlife Research Center, 4101 LaPorte Avenue, Fort Collins, Colorado, 80521",
"Ontario Ministry of Natural Resources and Forestry, 2140 East Bank Drive, Peterborough, Ontario, K9L 0G2, Canada",
"PCR",
"School of the Environment, Washington State University, 100 Dairy Road, Pullman, Washington, 99164",
"USGS:5d36fbfce4b01d82ce8a6cbf",
"Upper Midwest Environmental Sciences Center, 2630 Fanta Reed Road, La Crosse, Wisconsin, 54603",
"Whitney Genetics Laboratory, 555 Lester Avenue, Onalaska, Wisconsin, 54650",
"biota",
"detection",
"eDNA",
"invasive species"
]
|
| modified | 2022-04-28T00:00:00Z |
| publisher |
{
"name": "U.S. Geological Survey",
"@type": "org:Organization"
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|
| spatial | -180.0000, -90.0000, 180.0000, 90.0000 |
| theme |
[
"Geospatial"
]
|
| title | Reporting the limits of detection (LOD) and quantification (LOQ) for environmental DNA assays: Data |
