RR-1 and RR-3 mouse liver transcriptomics with and without ERCC control RNA spike-ins
Proper interpretation of RNA sequencing data requires an understanding of assay sensitivity and sources of variability. To this end, the External RNA Control Consortium (ERCC) developed a standard set of 92 poly-adenylated RNA transcripts that are orthogonal to mammalian RNA that can be added to RNA extracts before library generation and sequencing. The presence of these RNA standards at known ratios improves interpretation of RNA sequencing datasets. To test the utility of the ERCC RNA controls, total RNA extracted from mouse livers from the Rodent Research 1 (flight, ground control, basal control and vivarium control groups) and Rodent Research 3 (flight, ground control and basal control groups) missions were sequenced with and without the ERCC control RNA (RR-3 liver samples without ERCC control have been sequenced previously and the dataset is released in the GLDS-137). To allow comparison within and between groups, ERCC Mix 1 or Mix 2 were added to half of the samples from each group, respectively.
Complete Metadata
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| description | Proper interpretation of RNA sequencing data requires an understanding of assay sensitivity and sources of variability. To this end, the External RNA Control Consortium (ERCC) developed a standard set of 92 poly-adenylated RNA transcripts that are orthogonal to mammalian RNA that can be added to RNA extracts before library generation and sequencing. The presence of these RNA standards at known ratios improves interpretation of RNA sequencing datasets. To test the utility of the ERCC RNA controls, total RNA extracted from mouse livers from the Rodent Research 1 (flight, ground control, basal control and vivarium control groups) and Rodent Research 3 (flight, ground control and basal control groups) missions were sequenced with and without the ERCC control RNA (RR-3 liver samples without ERCC control have been sequenced previously and the dataset is released in the GLDS-137). To allow comparison within and between groups, ERCC Mix 1 or Mix 2 were added to half of the samples from each group, respectively. |
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| identifier | 10.26030/rwyp-9325 |
| keyword |
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| landingPage | https://osdr.nasa.gov/bio/repo/data/missions/SpaceX-4;https://osdr.nasa.gov/bio/repo/data/missions/SpaceX-8 |
| modified | 2025-08-21 |
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"Biological and Physical Sciences"
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|
| title | RR-1 and RR-3 mouse liver transcriptomics with and without ERCC control RNA spike-ins |
