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S-Layer Nanosheet Binding of Zn and Gd
This data characterizes binding of Zn2+ and Gd3+ to engineered nanosheets at 40C and in a brine solution. The engineered nanosheets are composed of surface-layer (S-layer) proteins which form 2 D crystalline sheets and display Zn2+- or Gd3+-binding domains on these sheets. Their ability to bind Zn2+ is compared to S-layer nanosheets that do not contain Zn2+-binding domains. We found that the purification method of these nanosheets was a critical determinant of their function and thus have provided data on the binding from two different purification methods.
A key distinction of this dataset from other datasets is that the engineered nanosheets were expressed and purified from E. coli grown at 37C as described in (Kinns, 2010; Howorka, 2000),
References:
Kinns, H., et al. Identifying assembly-inhibiting and assembly-tolerant sites in the SbsB S-layer protein from Geobacillus stearothermophilus. Journal of Molecular Biology, 2010. 395(4): p. 742-753.
Howorka, S., et al. Surface-accessible residues in the monomeric and assembled forms of a bacterial surface layer protein. Journal of Biological Chemistry, 2000. 275(48): p. 37876-37886.
Complete Metadata
| @type | dcat:Dataset |
|---|---|
| accessLevel | public |
| bureauCode |
[
"019:20"
]
|
| contactPoint |
{
"fn": "Caroline Ajo-Franklin",
"@type": "vcard:Contact",
"hasEmail": "mailto:cajo-franklin@lbl.gov"
}
|
| dataQuality |
true
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| description | This data characterizes binding of Zn2+ and Gd3+ to engineered nanosheets at 40C and in a brine solution. The engineered nanosheets are composed of surface-layer (S-layer) proteins which form 2 D crystalline sheets and display Zn2+- or Gd3+-binding domains on these sheets. Their ability to bind Zn2+ is compared to S-layer nanosheets that do not contain Zn2+-binding domains. We found that the purification method of these nanosheets was a critical determinant of their function and thus have provided data on the binding from two different purification methods. A key distinction of this dataset from other datasets is that the engineered nanosheets were expressed and purified from E. coli grown at 37C as described in (Kinns, 2010; Howorka, 2000), References: Kinns, H., et al. Identifying assembly-inhibiting and assembly-tolerant sites in the SbsB S-layer protein from Geobacillus stearothermophilus. Journal of Molecular Biology, 2010. 395(4): p. 742-753. Howorka, S., et al. Surface-accessible residues in the monomeric and assembled forms of a bacterial surface layer protein. Journal of Biological Chemistry, 2000. 275(48): p. 37876-37886. |
| distribution |
[
{
"@type": "dcat:Distribution",
"title": "Gd-Binding S-Layers.xlsx",
"format": "xlsx",
"accessURL": "https://gdr.openei.org/files/951/Gd-binding-S-layers.xlsx",
"mediaType": "application/vnd.openxmlformats-officedocument.spreadsheetml.sheet",
"description": "This data set characterizes binding of Gd3+ to engineered nanosheets at 40C. The engineered nanosheets are composed of surface-layer (S-layer) proteins which form 2 D crystalline sheets and display Gd3+-binding domains on these sheets. Their ability to bind Gd3+ is compared to S-layer nanosheets that do not contain Gd3+-binding domains."
},
{
"@type": "dcat:Distribution",
"title": "Zn-Binding S-Layers.xlsx",
"format": "xlsx",
"accessURL": "https://gdr.openei.org/files/951/Zn-binding-S-layers.xlsx",
"mediaType": "application/vnd.openxmlformats-officedocument.spreadsheetml.sheet",
"description": "This data set characterizes binding of Zn2+ to engineered nanosheets at 40C and in a brine solution. The engineered nanosheets are composed of surface-layer (S-layer) proteins which form 2 D crystalline sheets and display Zn2+-binding domains on these sheets. Their ability to bind Zn2+ is compared to S-layer nanosheets that do not contain Zn2+-binding domains."
}
]
|
| DOI | 10.15121/1372392 |
| identifier | https://data.openei.org/submissions/7119 |
| issued | 2016-04-15T06:00:00Z |
| keyword |
[
"Gadolinium",
"S-layer",
"binding",
"brine study",
"chemistry",
"energy",
"gd3",
"geothermal",
"ion",
"metal-binding domain",
"mineral recovery",
"nanosheet",
"synthetic biology",
"zinc",
"zn2"
]
|
| landingPage | https://gdr.openei.org/submissions/951 |
| license | https://creativecommons.org/licenses/by/4.0/ |
| modified | 2020-01-09T00:11:19Z |
| programCode |
[
"019:006"
]
|
| projectLead | Holly Thomas |
| projectNumber | FY15 AOP 2514 |
| projectTitle | Engineering Thermophilic Microorganisms to Selectively Extract Strategic Metals from Low Temperature Geothermal Brines |
| publisher |
{
"name": "Lawrence Berkeley National Laboratory",
"@type": "org:Organization"
}
|
| spatial |
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|
| title | S-Layer Nanosheet Binding of Zn and Gd |
